胸苷磷酸化酶在胃癌组织中的表达及临床意义

目的 :探讨胸苷磷酸化酶 (thymidinephosphorylase ,TP) /血小板衍生内皮细胞生长因子 (platelet deriveden dothelialcellgrowthfactor,PD ECGF)和微血管密度 (microvesseldensity,MVD)在胃癌中的表达及临床意义。方法 :应用免疫组化SP法 ,检测 12 0例人胃癌组织中TP/PD ECGF蛋白表达及微血管密度 ,分析TP/PD ECGF和MVD及其与胃癌临床病理因素及预后的关系。结果 :TP阳性组MVD值显著高于TP阴性组 (P <0 .0 1) ,TP表达与肿瘤的血行转移、淋巴结转移、浸润深度密切相关 ,TP阳性组肝转移率明显高于TP阴性组。MVD与胃癌的肿块大小、TMN分期、淋巴结转移、浸润深度、血行转移密切相关 (P <0 .0 1) ;而与肿瘤分化无关 (P >0 .0 5 )。结论 :TP/PD ECGF与胃癌的血管生成密切相关 ,对胃癌的生长、浸润、转移起促进作用。TP/PD ECGF与MVD可作为反映胃癌生物学行为的指标 ,同时也是判断预后和指导辅助治疗的有效指标。

Correlation between Thymidine Phosphorylase Expression and Hepatic Metastasis in Human Gastric Cancer

Objective: To study the expression of thymidine phosphorylase(TP)/platelet-derived endothelial cell growth factor(PD-ECGF),microvessel density (MVD) in gastric carcinoma tissues and their clinical signification. Methods: TP/PD-ECGF protein expression and microvessel density(MVD) in 120 samples of gastric carcinoma was examined by means of immunohistochemical staining S-P method,and the relationship of TP/PD-ECGF、MVD with their clinical pathological characteristics and the prognosis of gastric cancers was analysed. Results: The MVD was significantly higer in TP-postive tumours than in TP-negative tumours. The TP expression closely correlated with depth of invasion,lymph node metastasis and venous invasion of tumours,and the frequency of hepatic metastasis was significantly higher in patients with TP-positive tumours than that in patients with TP-negative tumours. MVD positively correlated with size of tumour,Dukes period,lymph node metastasis,venous invasion and depth of invasion(P<0.01),but showed no significant correlation with the histologic type(P>0.05). Conclusion: TP/PD-ECGF highly correlates with angiogenesis of gastric carcinoma,and promotes growth invasion and hepatic metastasis of gastric carcinoma. TP/PD-ECGF expression or MVD may be one of the predictors of the biological behaviors of gastric carcinoma,and both may serve as the prognostic factors and be helpful for decision on the therapy.