Isolation of Trypanosoma brucei variant specific antigen mRNA by immunoprecipitation of polysomes |
| |
| Authors: | John S Cordingley Mervyn J Turner |
| |
| Institution: | Medical Research Council Biochemical Parasitology Unit, The Molteno Institute, Downing Street, Cambridge CB2 3EE, U.K. |
| |
| Abstract: | The mRNA encoding the variant specific antigen of Trypanosoma brucei has been prepared by immunoprecipitation of polysomes. Polysomes carrying the variant specific antigen account for approx. 3% of the total polysomes. The mRNA thus produced is active in the mRNA dependent rabbit reticulocyte lysate in vitro protein synthesis system and directs the synthesis of a polypeptide of 60 000 daltons which co-migrates both with 125I-labelled purified variant specific antigen and with antigen immunoprecipitated from reticulocyte lysate charged with total polyadenylated mRNA from the same clone. The mRNA is being used both to prepare cDNA clones and to prepare high specific radioactivity cDNA to be used to screen a gene bank for clones containing variant specific antigen coding sequences. |
| |
| Keywords: | Immunoprecipitation Polysomes mRNA Variant surface antigen In vitro protein synthesis VSA variant surface antigens SDS sodium dodecyl sulphate GAR-PAB |
| 本文献已被 ScienceDirect 等数据库收录！ |
