| 紫外线照射对恶性肿瘤患者粒—单造血祖细胞集落形成和细胞活？… |
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| 引用本文： | 岳寿伟 侯怀水. 紫外线照射对恶性肿瘤患者粒—单造血祖细胞集落形成和细胞活？…[J]. 中华理疗杂志, 2000, 23(2): 96-98 |
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| 作者姓名： | 岳寿伟 侯怀水 |
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| 作者单位： | 山东医科大学附属医院 |
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| 摘 要： | 目的 研究短波紫外线照射对恶性肿瘤患者粒－单造血祖细胞集落形成和细胞活力的影响。方法 应用０．５０、１００，３００、４００，８００ｍＪ／ｃｍ＾２的ＵＶＣ照射应用干细胞生长因子动员后白细胞外周血单个核细胞（ＰＢＭＮＣ），观察７ｄ后粒－单系造血祖细胞集落（ＣＦＵ－ＧＭ）以及于ＵＶＣ照射后１，３，５，７ｄ观察细胞活力。结果 ５０，１００，２００，４００，８００ｍＪ／ｃｍ＾２ＵＶＣ照射后ＣＦＵ－ＧＭ集落个
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| 关 键 词： | 恶性肿瘤 紫外线照射 造血干细胞 测定 |
Effect of ultraviolet C irradiation on CFU-GM and cellular viability in vitro |
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| YUE Shouwei,HOU Huaishui,SHI Qing,et al.. Effect of ultraviolet C irradiation on CFU-GM and cellular viability in vitro[J]. Chinese Journal of Physical Therapy, 2000, 23(2): 96-98 |
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| Authors: | YUE Shouwei HOU Huaishui SHI Qing et al. |
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| Affiliation: | YUE Shouwei,HOU Huaishui,SHI Qing,et al.Affiliated Hospital of Shandong Medical University,Jinan 250012 |
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| Abstract: | Objective To determine the effect of ultraviolet C(UVC) irradiation on CFU-GM(colony forming unit/graulo-monocyte) and cellular viability in leukemia.Methods Peripheral blood monocyte was irradiated by UVC(25.37 nm) in 0,50,100,300,400,800 mJ/cm 2 respectively.The CFU-GM was observed at 7th day after UVC irradiation,the cellular viability was determined after 1,3,5 and 7 days of UVC.Comparison was performed between the experimental group and control group.Results The study showed that CFU-GM was obviously reduced at post-UVC irradiation(P<0.01),which was in pace with increase of UV dose.However,the drop of cellular viability was less than CFU-GM relatively.Conclusion It is suggested that UVC irradiation can decrease the CFU-GM,but it does not kill the cells directly.The alteration of cell membrane receptor radiated by UVC may impact the cell functions possibly. |
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| Keywords: | Ultraviolet rays Hemotopoietic cell growth factors Tumor stem cell assay |
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