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| Construction of HA-1-DC Nucleic-acid Vaccine and Induction of Specific Cytotoxic T Lymphocytes | |
| 引用本文： | 汪涯雅,张东华,胡金梅,刘文励,周红升,张路,刘丹,黄振倩,谭获. Construction of HA-1-DC Nucleic-acid Vaccine and Induction of Specific Cytotoxic T Lymphocytes[J]. 华中科技大学学报(医学英德文版), 2007, 27(3): 261-264. DOI: 10.1007/s11596-007-0311-y |
| 作者姓名： | 汪涯雅 张东华 胡金梅 刘文励 周红升 张路 刘丹 黄振倩 谭获 |
| 作者单位： | Tumor Blood Center First Affiliated Hospital Guangzhou Medical College，Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Internal Medicine Songzi People's Hospital，Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Tumor Blood Center First Affiliated Hospital Guangzhou Medical College，Tumor Blood Center First Affiliated Hospital Guangzhou Medical College，Tumor Blood Center First Affiliated Hospital Guangzhou Medical College，Guangzhou 510230 China，Wuhan 430030 China，Songzi 434200 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Guangzhou 510230 China，Guangzhou 510230 China，Guangzhou 510230 China |
| 摘 要： | An HA-1-DC nucleic-acid vaccine was constructed to induce anti-leukemia effect after hematopoietic stem cell transplantation (HSCT). DCs were generated from HSCT donors in vitro, and its immunologic activity was assayed by using flow cytometry and mixed lymphocytes reaction. HA-1 gene was electroporated into the cultured DCs to construct a DC nucleic-acid vaccine. After transfection for 48 h, the expression of HA-1 protein could be detected by using Western blot. The DCs were cultured with syngenic lymphocytes to induce specific cytotoxic T lymphocytes (CTLs). The cytoxicity of the CTLs was detected by LDH assay. The results showed that The DCs derived from peripheral blood monocytes (PBMCs) expressed the phenotype of DCs, and were effective in stimulating proliferation of the allogenic lymphocytes. After electroporating for 48-h, HA-1 protein was detected by using Western blot. The cytotoxity of inducing CTLs was higher than the control group. It was suggested that minor histocompatibility antigen HA-1 could be considered as a target of immunotherapy against leukemia after HSCT. |
| 收稿时间： | 2006-12-22 |
Construction of HA-1-DC nucleic-acid vaccine and induction of specific cytotoxic T lymphocytes |
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| Wang Yaya,Zhang Donghua,Hu Jinmei,Liu Wenli,Zhou Hongsheng,Zhang Lu,Liu Dan,Huang Zhenqian,Tan Huo. Construction of HA-1-DC nucleic-acid vaccine and induction of specific cytotoxic T lymphocytes[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2007, 27(3): 261-264. DOI: 10.1007/s11596-007-0311-y | |
| Authors: | Wang Yaya Zhang Donghua Hu Jinmei Liu Wenli Zhou Hongsheng Zhang Lu Liu Dan Huang Zhenqian Tan Huo |
| Affiliation: | 1. Tumor Blood Center, First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510230, China 2. Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China 3. Department of Internal Medicine, Songzi People's Hospital, Songzi 434200,China |
| Abstract: | An HA-1-DC nucleic-acid vaccine was constructed to induce anti-leukemia effect after hematopoietic stem cell transplantation (HSCT). DCs were generated from HSCT donors in vitro, and its immunologic activity was assayed by using flow cytometry and mixed lymphocytes reaction. HA-1 gene was electroporated into the cultured DCs to construct a DC nucleic-acid vaccine. After transfection for 48 h, the expression of HA-1 protein could be detected by using Western blot. The DCs were cultured with syngenic lymphocytes to induce specific cytotoxic T lymphocytes (CTLs). The cytoxicity of the CTLs was detected by LDH assay. The results showed that The DCs derived from peripheral blood monocytes (PBMCs) expressed the phenotype of DCs, and were effective in stimulating proliferation of the allogenic lymphocytes. After electroporating for 48-h, HA-1 protein was detected by using Western blot. The cytotoxity of inducing CTLs was higher than the control group. It was suggested that minor histocompatibility antigen HA-1 could be considered as a target of immunotherapy against leukemia after HSCT. |
| Keywords: | dendritic cell HA-1 nucleic-acid vaccine hematopoietic stem cell transplantation cytotoxic T lymphocyte |
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