铬结合物在铬中毒性肝损害中的作用

目的了解各种铬结合物与肝损害的关系。方法在铬中毒性肝损害的大鼠模型中，采用ＳｅｐｈａｄｅｘＧ５０凝胶层析技术，对肝中铬结合物进行分离和测定。结果铬染毒大鼠肝组织中，除可检出高分子铬结合物（ＨＭＷＣｒ，相对分子质量（ＭＷ）７００００～１２５００）及低分子铬结合物（ＬＭＷＣｒ，ＭＷ１２５００～５００）外，还发现对照组未检出的小分子铬结合物（ＭＭＷＣｒ，ＭＷ＜５００）。随染毒时间延长，肝损害加重，三者肝中分布变化明显：ＨＭＷＣｒ由２２．２％增至３７．０％；ＭＭＷＣｒ由２．２％增至５．０％；ＬＭＷＣｒ则从７５．６％降至５８．０％。结论ＬＭＷＣｒ可能与铬的解毒有关；ＭＭＷＣｒ的出现并增多反映了机体形成ＬＭＷＣｒ能力的紧张；ＭＭＷＣｒ可能是攻击靶器官的主要活性因素。

Study on the role of chromiumbinding substances in chromiuminduced hepatic damage

Objective To study the role of chromiumbinding substances in chromiuminduced hepatic damage. Methods A subacute chromium intoxicated rat model was made and chromiumbinding substances in liver were isolated and measured by sephadex G50 column chromatography and UVAAS. Results Only a high molecular weight chromiumbinding substance (HMWCr,MW700 00012 500) and a low molecular weight chromiumbinding substance (LMWCr,MW12 500500) could be detected in normal animals.But in chromiumexposed animals,a new minimal molecular weight chromiumbinding substance (MMWCr,MW<500) was found in addition to the above constituents.As the exposure time prolonged,hepatic damage aggravated and the distribution of three Crbinding substances was as follows:HMWCr from 22.2% to37.0%,MMWCr from 2.2% to 5.0%,LMWCr from 75.6% to 58.0%. Conclusion LMWCr play a main role in chromium detoxification.The appearance and increase of MMWCr reflected on the insufficiency of LMWCr formation.The MMWCr should be a major active factor on pathogenesis of Crintoxication.