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| 用睾丸细胞共培养探讨吡哆醇对大鼠睾丸细胞的毒性 | |
| 引用本文： | 黄厚今,王瑞淑,徐维光,杨青. 用睾丸细胞共培养探讨吡哆醇对大鼠睾丸细胞的毒性[J]. 中华劳动卫生职业病杂志, 1999, 17(1): 20-22 |
| 作者姓名： | 黄厚今 王瑞淑 徐维光 杨青 |
| 作者单位： | 1. 563003,遵义医学院 2. 华西医科大学营养与食品卫生学教研室 3. 华西医科大学劳动卫生学教研室 |
| 摘 要： | 目的探讨吡哆醇（ＰＮ）对大鼠睾丸的体外毒性。方法采用在Ｗｉｌｉａｍｓ方法的基础上改进的Ｓｅｒｔｏｌｉ ｇｅｒｍ细胞共培养系统,观察ＰＮ在不同剂量和接触时间对培养细胞的作用。结果脱落生精细胞数随ＰＮ浓度的增高和接触时间的延长而增加,并有明显的剂量—效应和时间—效应关系。同时,还观察到Ｓｅｒｔｏｌｉ细胞骨架出现松弛、回缩等效应。结论ＰＮ对大鼠生精细胞的体外效应反映了其对Ｓｅｒｔｏｌｉ细胞的损害。睾丸细胞共培养方法对探讨ＰＮ对大鼠睾丸的毒性作用具有实用价值。 |
| 关 键 词： | 吡哆醇 睾丸 大鼠 Sertoli-germ细胞共培养 |
Effects of pyridoxine on rat testis cells in Sertoli-germ cell co-culture system |
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| Huang Houjin,Wang Ruishu,Xu Weiguang,et al.. Effects of pyridoxine on rat testis cells in Sertoli-germ cell co-culture system[J]. Chinese journal of industrial hygiene and occupational diseases, 1999, 17(1): 20-22 | |
| Authors: | Huang Houjin Wang Ruishu Xu Weiguang et al. |
| Affiliation: | Huang Houjin,Wang Ruishu,Xu Weiguang,et al.Zunyi Medical College,Zunyi 563003 |
| Abstract: | Objective To understand the toxic effects of pyridoxine(PN) on rat testis cells in vitro . Method In a Sertoli germ cells co culture system modified from Williams method,the toxic effects of PN at different concentrations and exposed duration were observed. Results The detachment of germ cells showed marked dose response and time response relationships.Meanwhile,the characteristic of loosing and retracting skeleton in the Sertoli cells was found. Conclusion The effects produced by PN in vitro may reflect damage to Sertoli cells,and testicular cells co culture could be of value for the study of underlying mechanisms of toxic effects of PN on rat testis. |
| Keywords: | Pyridoxine Testis Rat Sertoli germ cell co culture |
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