直流电对鳞状细胞癌Tca-83细胞系抑杀作用的体外研究

目的:探讨直流电对人舌鳞状细胞癌Tca-83细胞系的抑杀作用。方法:对体外培养的人舌鳞状细胞癌细胞系Tca-83采用不同的电处理参数进行了直流处理。观察处理后癌细胞的形态、结构、生长情况、生物学特性;检测电处理后培养液的酸碱度、电解质的改变情况。结果:直流电处理对舌鳞状细胞癌有明显的杀伤及抑制作用。在电压一定的情况下增加电量可提高抑杀效率。电处理后的培养液pH,电解质,有机成分发生较大改变,其对癌细胞的生长有一定的抑制作用。电处理后未被杀死的癌细胞增殖能力也明显减低。结论:直流电处理可通过电解、电泳、电渗作用直接杀伤舌癌细胞,亦可通过改变细胞生长的外环境抑制舌癌细胞修复、生长、增殖

Cytocidal and inhibitory effects of direct current on human tongue squa mous cell carcinoma cell line Tca83

Objective: To study the cytocidal and inhibitory effects of direct currents on human tongue squamous cell carcinoma cell line(Tca 83). Methods:Tca 83 cells were treated with direct currents with different parameters. The effects of the treatment were evaluated by morphological observation, MTT assay and detection of pH and electrolyte in the treated culture medium.Results:Direct current could kill and inhibit the growth of Tca 83 cells, after the electric treatment, modify of pH and electrolyte of culture medium could also kill and inhibit the recover and growth of the cells. Conclusion:Direct current treatment can kill the cells of tongue squamous cell carcinoma directly by electrolysis, electrophoresis and eletroendosmosis, and inhibit the cell growth indirectly by environment alteration.