PCR方法快速检测一些常见医学真菌

目的 为选择一种快速、敏感和特异的方法来检测临床感染的真菌。方法 选取真菌１８ＳｒＤＮＡ序列中两个片段设计引物，对１２种菌株进行ＰＣＲ扩增，均扩增出长约３１０ｂｐ产物。对一些临床常见细菌扩增均无反应。对酶母相真菌白念珠菌、新生隐球菌进行敏感性实验检测。结果 发现二者ＰＣＲ最小检出量均为菌体数达每个反应体系１０＾３。本实验过程总耗时３６ｈ以内。结论 本实验方法可快速特异性检测一些常见医学真菌。

Rapid Detection of Medical Important Fungi by PCR

Objective To develop a method with high sensitivity, speciality and rapidity to detect clinical important fungi.Methods Two fragments of 18S ribosomal DNA were selected as universal primers in this PCR amplification.A 310bp product was obtained for all twelve general and species of fungi.Otherwise, a variety of pathogenic bacteria gave no cross-hybridization under the same condition of reaction. Sensitivity tests were performed on two yeast form fungi, C albicans and C . neoformans.Result The least counts could be detected both were limited at 10 3.This study reported the detection fungi within 36 hours.Conclusion The test could detect medical important fungi rapidly and specially.