| 白细胞介素6基因启动子区多态性与冠状动脉疾病的相关性研究 |
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| 引用本文： | 刘永超,段宗明,张明娟.白细胞介素6基因启动子区多态性与冠状动脉疾病的相关性研究[J].临床荟萃,2011,26(14):1200-1203. |
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| 作者姓名： | 刘永超 段宗明 张明娟 |
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| 作者单位： | 西安交通大学医学院第二附属医院综合内科,陕西西安,710004 |
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| 摘 要： | 目的 探讨白细胞介素6(interleukin 6,IL-6)基因启动子区-174G/C、-572C/G和-634C/G多态性对冠状动脉疾病(coronary heart disease,CHD)的发病易感性.方法 严格按照诊断标准,选取无亲缘关系的CHD患者126例(CHD组)及健康对照组150例提取基因组DNA,应用聚合酶链反应-限制性片段长度多态性(PCR-polymorphism,Restriction Fragment,PCR-RFLP)技术检测-174 G/C、-572 C/G和-634 C/G 3个多态性位点的基因型;采用HaploView4.0及SPSS11.5软件分析各位点基因型、等位基因频率及组间差异.结果 -572C/G位点及-634C/G位点的基因型频率分布在CHD组与正常对照组差异均有统计学意义(P＜0.05),CHD组-572C/G位点的等位基因G频率显著高于正常对照组(P＜0.05),-634C/G位点的等位基因G频率显著高于正常对照组(P＜0.05).连锁不平衡检验结果显示,IL-6基因这3个位点处于不连锁状态,D'＜0.5.结论 IL-6基因-174G/C和-572C/G多态性可能与CHD有关,携带有-572C/G和-634C/G多态性位点G等位基因的个体可能更容易患CHD.
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| 关 键 词： | 冠状动脉疾病 白细胞介素6 启动区(遗传学) 多态性 限制性片段长度 |
Association between polymorphisms in interleukin-6 gene promoter region and coronary artery disease |
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| LIU Yong-chao
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DUAN Zong-ming
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ZHANG Ming-juan.Association between polymorphisms in interleukin-6 gene promoter region and coronary artery disease[J].Clinical Focus,2011,26(14):1200-1203. |
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| Authors: | LIU Yong-chao DUAN Zong-ming ZHANG Ming-juan |
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| Institution: | (Department of General Internal Medicine,the Second Affiliated Hospital of Xi＇an Jiaotong University School of Medicine,Xi′an 710004,China) |
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| Abstract: | Objective To study a possible association between the-174G/C,-572C/G and-634C/G of the interleukin-6(IL-6) gene promoter region polymorphisms and coronary heart disease(CHD).Methods Genomic DNA was isolated from the venous blood leukocytes of 126 unrelated patients with CHD(study group) and 150 healthy unrelated individuals(control group).Polymorphisms of-174G/C,-572C/G and-634C/G were genotyped by PCR-restriction fragment length polymorphisms(PCR-RFLP).Genotype and allele frequencies were analyzed by Haplo... |
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| Keywords: | coronary disease interleukin-6 promoter(genetic) polymorphism restriction fragment length polymorphisms |
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