Transplantation of cultured human spinal cord cells into the rat motor cortex: use of Phaseolus vulgaris leucoagglutinin as a cell marker |
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| Authors: | H Kamo S U Kim P L McGeer M Araki H Tomimoto H Kimura |
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| Affiliation: | 1. Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver, B.C. Canada;2. Division of Neurology, Department of Medicine, University of British Columbia, Vancouver, B.C. Canada;3. Jichi Medical School, Tochigi-Ken Japan;4. Department of Neurology, Kyoto University School of Medicine, Kyoto Japan;5. Department of Anatomy, Shiga University of Medical Science, Shiga Japan;1. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;2. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;3. Department of Rehabilitation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;4. Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;5. Waisman Center, Department of Neuroscience and Department of Neurology, University of Wisconsin, Madison, WI, USA;6. Program in Neuroscience & Behavioral Disorders, Duke-NUS Medical School, Singapore, Singapore;7. Stem Cell Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;8. Key Laboratory of Neurological Diseases of Chinese Ministry of Education, the School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;1. Department of Rehabilitation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;2. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;3. School of Mechanical Engineering, Hubei University of Technology, Wuhan 430068, China;4. Waisman Center, Department of Neuroscience and Department of Neurology, University of Wisconsin, Madison, WI, USA;5. Program in Neuroscience & Behavioral Disorders, Duke-NUS Medical School, Singapore, Singapore |
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| Abstract: | ![]()
Successful transplantations have been made of cultured explants of human fetal spinal cord into surgically created cavities in the motor cortical area of non-immunosuppressed young adult rats. The cultured cells were marked by brief incubation with Phaseolus vulgaris leucoagglutinin (PHA) just prior to transplantation. Following sacrifice of the rats 1.5 months later, PHA immunohistochemistry clearly outlined the demarcation zone of the explants. The transplanted neurons possessed long, somewhat tortuous fibers with occasional varicosities, as well as some thick processes. These findings extend our previous studies in which it was shown that cultured human fetal adrenal medulla and sympathetic ganglia cells could be successfully transplanted to non-immunosuppressed rat brain. They also suggest that PHA may be a valuable marker for transplanted cells at least for 1.5 months post-transplantation. |
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