| 携带HSV－tk基因的人肝癌特异性逆转录病毒载体的构建 |
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| 引用本文： | 高军,曹广文,戚中田,仇小芳,吴宗娣,杜平,杨文国,崔龙. 携带HSV－tk基因的人肝癌特异性逆转录病毒载体的构建[J]. 第二军医大学学报, 1996, 0(2) |
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| 作者姓名： | 高军 曹广文 戚中田 仇小芳 吴宗娣 杜平 杨文国 崔龙 |
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| 作者单位： | 第二军医大学基础医学部微生物学教研室，第二军医大学长海医院普通外科 |
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| 摘 要： | 构建携带单纯疱疹病毒ｔｋ基因（ＨＳＶ－ｔｋ）的人肝癌特异性逆转录病毒载体。方法和结果：切除逆转录病毒载体ｐＭＮＳＭ内部的ＳＶ４０启动子使之成为ｐＭＮＭ；从真核表达载体ｐＢＰＧＫ－ｔｋ质粒中游离带有磷酸甘油酸激酶基因（ｐｇｋ）启动子调控的ＨＳＶ－ｔｋ片段，克隆到ｐＭＮＭ的ＰＬ位点上，构建成普通型ＰＭＮｐ－ｔｋ逆转录病毒载体；从ｐＧＥＭ．７Ｚ－ＡＦＰｅ质粒中游离人甲胎蛋白基因增强子核心序列，克隆到ｐＭＮｐ－ｔｋ的ｐｇｋ启动子上游，构建成人肝癌特异型ｐＭＮＡＰ－ｔｋ逆转录病毒载体。结论：该载体对肝癌特异性前药转换基因治疗有重要意义。
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| 关 键 词： | 单纯疱疹病毒；胸苷激酶基因；人甲胎蛋白；增强子；逆转录病毒载体；基因治疗 |
Construction of retroviral vector carrying HSV-tk gene under control of human AFP enhancer core sequence and human pgk promoter |
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| Gao Jun,Cao Guangwen,Qi Zhongtian,Qiu Xiaofang,Wu Zongdi,Du Ping,Yang Wenguo,Cui Long. Construction of retroviral vector carrying HSV-tk gene under control of human AFP enhancer core sequence and human pgk promoter[J]. Former Academic Journal of Second Military Medical University, 1996, 0(2) |
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| Authors: | Gao Jun Cao Guangwen Qi Zhongtian Qiu Xiaofang Wu Zongdi Du Ping Yang Wenguo Cui Long |
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| Abstract: | Objective:Construction of retroviral vector carrying HSV-tk gene under control of human a-fetoprotein(AFP) enhancer core sequence and human pgk promotor.Methods and Results:Internal SV40promoter was deleted from retroviral vector pMNSM to construct pMNM. HSV-tk gene driven by pgk promoter was released from an eukaryotic expression vector pBPGK-tk,and inserted into polylinker site of pMNM to construct pMNP -tk retroviral vector.Human AFP gene enhancer core sequence released from pGEM.7Z-AFPe plasmid was inserted into the immediate upstream of pgk promoter of pMNP-tk vector.Construction of hepatoma-specific retroviral vector pMNAP-tk was completed.Conclusion:The vector is of great significance for hepatoma-specific prodrug transformation gene therapy. |
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