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| 磁共振报告基因magA的慢病毒载体质粒构建及体外表达 | |
| 引用本文： | 秦勇,蔡金华,郑鹤琳,刘官信,王世一,刘波. 磁共振报告基因magA的慢病毒载体质粒构建及体外表达[J]. 第三军医大学学报, 2011, 33(13): 1346-1349 |
| 作者姓名： | 秦勇 蔡金华 郑鹤琳 刘官信 王世一 刘波 |
| 作者单位： | 1. 400014,重庆,重庆医科大学附属儿童医院:放射科;400014,重庆,重庆医科大学附属儿童医院:儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地 2. 重庆医科大学附属儿童医院:放射科,重庆,400014 3. 重庆医科大学附属儿童医院:儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆,400014 |
| 基金项目： | 重庆市自然科学基金(CSTC2009JJ0305)~~ |
| 摘 要： | 目的 构建携带磁共振报告基因magA的慢病毒载体质粒,初步检测其体外转铁效应.方法 采用人工DNA合成技术合成目的 基因magA,DNA重组技术将magA基因连接入慢病毒表达载体质粒pLenti-EGFP,酶切及DNA测序鉴定重组质粒pLenti-EGFP/magA的准确性.包装、包膜及重组质粒共转染293FT细胞包装... |
| 关 键 词： | magA 报告基因 磁共振成像 慢病毒载体 |
Construction of recombinant lentivirus expression vector carrying MagA and its in vitro expression |
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| Qin Yong,Cai Jinhua,Zheng Helin,Liu Guanxin,Wang Shiyi,Liu Bo. Construction of recombinant lentivirus expression vector carrying MagA and its in vitro expression[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(13): 1346-1349 | |
| Authors: | Qin Yong Cai Jinhua Zheng Helin Liu Guanxin Wang Shiyi Liu Bo |
| Affiliation: | Qin Yong1,2,Cai Jinhua1,Zheng Helin1,Liu Guanxin2,Wang Shiyi2,Liu Bo1(1Department of Radiology,2Key Laboratory of Child Development and Disorders Cofounded by Chongqing and Ministry of Education,Key Laboratory of Pediatrics of Chongqing,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders,Children's Hospital,Chongqing Medical University,Chongqing,400014,China) |
| Abstract: | Objective To construct a recombinant lentivirus expression vector encoding magA,a new MRI reporter gene,and to determine the iron-transporting effect of in vitro expressed magA.Methods The gene sequence of magA was synthesized by synthetic DNA technology,and then inserted into the lentivirus expression vector pLenti-EGFP by recombinant DNA technique.The recombinant lentivirus expression vector pLenti-EGFP/magA was verified by enzyme digestion and DNA sequencing.Then the recombinant lentivirus was packaged i... |
| Keywords: | magA reporter gene magnetic resonance imaging lentivirus vector |
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