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| 五味子糖浆中五味子醇甲含量测定 | |
| 引用本文： | 刘秋丽,左冬冬. 五味子糖浆中五味子醇甲含量测定[J]. 中医药信息, 2010, 27(3): 63-65 |
| 作者姓名： | 刘秋丽 左冬冬 |
| 作者单位： | 1. 哈药集团中药二厂,黑龙江,哈尔滨,150078 2. 黑龙江中医药大学附属第一医院,黑龙江,哈尔滨,150040 |
| 摘 要： | 目的:建立反相高效液相色谱法测定五味子糖浆中五味子醇甲含量的方法。方法:采用Kroma-silC18反相色谱柱(250mm×4.6mm5μm),流动相:甲醇-水(体积比60∶40);流速:1.0mL.min-1,检测波长:250nm。结果:五味子醇甲在0.164μg～0.656μg范围内呈良好的线性关系(r=0.9997),回归方程为:Y=0.000000395X+0.023080996。五味子醇甲平均回收率为100.32%(RSD=1.85%)。结论:本法简便、准确,可用于五味子糖浆中五味子醇甲的含量测定。 |
| 关 键 词： | 高效液相色谱法 五味子糖浆 五味子醇甲 |
Determination Method of Schizandrin in Wuweizi Syrup by RP-HPLC |
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| LIU Qiu-li,ZUO Dong-dong. Determination Method of Schizandrin in Wuweizi Syrup by RP-HPLC[J]. Information on Traditional Chinese Medicine, 2010, 27(3): 63-65 | |
| Authors: | LIU Qiu-li ZUO Dong-dong |
| Affiliation: | LIU Qiu -li1,ZUO Dong -dong2 (1. Harbin Pharmaceutical Group CO.,LTD Second Chinese Medicine Factory,Harbin 150078,China,2. The First Affilicated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China) |
| Abstract: | Objective：To establish the determination method of schizandrin in wuweizi syrup with RP-HPLC. Metheds：The samples were analyzed on a Kromasil C18 column （5 μm,4. 6mm × 250mm） using formaldehyde -water（60∶ 40,V/V） as the mobile phase at a flow rate of 1. 0mL.min -1,250nm as the detection wave-length. Results：Syringin showed good relationship at the range of 0. 164μg ～ 0. 656μg（r = 0. 9997）. The average recovery of syringin was100. 32% and RSD = 1. 85%. Conclusions：The method was simple,convenient and accurate,and can be used for content determination of syringin and syringaresinol in wujiashen Granule. |
| Keywords: | HPLC wuweizi syrup schizandrin |
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