丙型肝炎肝组织和血清HCV RNA含量及其关系的研究

目的 了解丙型肝炎肝组织和血清中丙型肝炎病毒（ＨＣＶ）ＲＮＡ的含量及其相互关系。方法 采用荧光定量聚合酶链反应（ＰＣＲ）检测２４例慢性丙型肝炎患者肝组织和血清ＨＣＶＲＮＡ含量。结果 肝组织中ＨＣＶＲＮＡ含量（１０＾８至１０＾１２拷贝／克）与血清病毒含量（１０＾５至１０＾９．２拷贝／毫升）呈显著正相关（Ｐ〈０．０１），每克感染肝组织ＨＣＶＲＮＡ含量高于每毫升血清的１０＾２至１０＾４拷贝。慢性活性肝炎

Study on the relationship of HCV RNA between serum and liver tissue of hepatitis C

Objective In order to study the amounts of the hepatitis C viral RNA in the serum associated with that in the liver tissue.Methods The concentrations of HCV RNA were detected in 24 patients with chronic hepatitis C by quantitative polymerase chain reaction which was based on a Amplisensor assay. Results The amounts of HCV RNA (10 5 to 10 9.2 copies/ml) in the serum correlated with that in the liver tissue (10 8 to 10 12 copies/g) ( P <0.01). One gram of the infected liver tissue contained 10 2 to 10 4 times as many copies of the viral RNA as that of 1ml serum. Liver tissue of chronic aggressive hepatitis contained significantly higher amounts of the viral RNA than those of chronic persistent hepatitis. There was a positive correlation between the titer of HCV RNA in the serum and ALT level. Conclusion These results suggested that a significant correlation between the quantity of HCV RNA in the liver and histological acti vity of chronic hepatitis C, and the serum HCV RNA levels could be considered as a marker for monitoring intrahepatic viral RNA.