安氟醚和异氟醚对肝脏缺血/再灌注损害的影响

目的 研究安氟醚和异氟醚预处理对肝脏缺血／再灌注损害的影响。方法 １８只家兔随机分３组，对照组阻断肝劝脉和门静脉血流形成肝缺血４５ｍｉｎ，开放再灌注１２０ｍｉｎ；安氟醚和异氟醚预处理组，于缺血再灌注前分别吸入１．６８％安氟醚和１．１５％异氟醚２０ｍｉｎ和药物清除１０ｍｉｎ。结果 缺血前，三组血清谷丙转氨酶（ＡＬＴ），谷草转氨酶（ＡＳＴ）、乳酸脱氢酶（ＬＤＨ）、碱性磷酸酶（ＡＬＰ）和谷氨酰酶（ＧＣＴ

Effects of preconditioning with enflurane and isoflurane on hepatic ischemia and reperfusion injury in rabbits

Objective To study the effects of the preconditioning with enflurane and isoflurane on hepatic ischemia and reperfusion injury Methods Eighteen rabbits were randomly allocated to receiving no pretrearment (control group, n=6),preconditioning with 1 68% enflurane (PE group,n=6),or preconditioning with 1 15% isoflurane(PI group,n=6), respectively, before undergoing 45 min of hepatic arterial and portal venous occlusions followed by 120 min of reperfusion The arterial blood samples were taken before the occlusion ,10 min and 120 min following reperfusion ,to measure the activities of serum alanine transminase (ALT), aspartate transminase (AST), lactic dehydrogenase (LDH), alkaline phosphatase (ALP) and gama glutaminase(GGT) using auto biochemical analyzer The liver histopathology were observed under light microscope Results Compared with those in control group , in PI and PE group the activities of serum ALT, AST, LDH, ALP and GGT were kept unchanged significantly before the ischemia (P>0 05); the increased degree of only AST activity reduced in PE group(P<0 05), others remained unchanged markedly in PI and PE group (P<0 05) 10 min following reperfusion; the increased degrees of all activities above reduced obviously in PI and PE group (P<0 05)120 min following reperfusion The injury degrees of liver histopathology in PI and PE groups were much less than that in control group Conclusions Preconditoning with enflurane and isoflurane can protect liver from ischemia and reperfusion injury to some extent