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| 一种改进的溶血素测定法 | |
| 引用本文： | 董波 秦凤华. 一种改进的溶血素测定法[J]. 军事医学科学院院刊, 1999, 23(2): 137-139 |
| 作者姓名： | 董波 秦凤华 |
| 作者单位： | 军事医学科学院放射医学研究所 |
| 摘 要： | 目的：改进溶血素测定方法。方法：在待测样品中加入Ｓｙｓｍｅｘ公司生产的快速溶血剂－Ⅱ，用半自动生化仪测Ｄ５４０，计算出样品半数溶血值ＨＣ５０。结果：用该方法测定联苯双酯、环磷酰胺和阿司匹林３种已知药物对绵羊红细胞（ＳＲＢＣ）致敏小鼠产生溶血素的影响，分别为无作用、抑制和增强，与以往文献报道的结果一致。结论：改进的溶血素测定法操作简单省时，可以用于药物筛选和药效评价。 |
| 关 键 词： | 溶血素测定法 药物筛选 免疫药物 |
An improved method for hemolysin assay |
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| Dong Bo,Qin Fenghua,Geng Yu,Xiong Guolin,Liu Xiaolan,Luo Qingliang,Chen Guozhi,Qiu Liling,Mao Bingzhi Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing. An improved method for hemolysin assay[J]. Bulletin of the Academy of Military Medical Sciences, 1999, 23(2): 137-139 | |
| Authors: | Dong Bo Qin Fenghua Geng Yu Xiong Guolin Liu Xiaolan Luo Qingliang Chen Guozhi Qiu Liling Mao Bingzhi Institute of Radiation Medicine Academy of Military Medical Sciences Beijing |
| Affiliation: | Dong Bo,Qin Fenghua,Geng Yu,Xiong Guolin,Liu Xiaolan,Luo Qingliang,Chen Guozhi,Qiu Liling,Mao Bingzhi Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850 |
| Abstract: | Objective: To improve the method for hemolysin assay. Methods: Quiklyser produced by Sysmex Corporation was added into the samples and 50% hemolysis value(HC50) was calculated by measuring D 540 with semiautomatic biochemical analyser. Results: Bifendate did not affect, cyclophosphamide inhibited and aspirin enhanced the production of hemolysin in the mice immunized by SRBC. These results are coincident with those reported by others. Conclusion: The improved method for hemolysin assay is simple and fast. It may be used to screen and evaluate drugs. |
| Keywords: | method for hemolysin assay drug screening |
| 本文献已被 CNKI 维普 等数据库收录！ | |