|
|||||
|
|
| 骨髓间充质干细胞来源成骨细胞培养体系中加入α-玉米赤霉醇干预后的骨保护素表达*** | |
| 引用本文： | 刘未华,段 斐,李少春,孙晓芳,郝华超,何 静,薛 娟,邓 鑫,罗雷雷,郭文龙. 骨髓间充质干细胞来源成骨细胞培养体系中加入α-玉米赤霉醇干预后的骨保护素表达***[J]. 中国组织工程研究, 2012, 16(6): 977-980. DOI: 10.3969/j.issn.1673-8225.2012.06.006 |
| 作者姓名： | 刘未华 段 斐 李少春 孙晓芳 郝华超 何 静 薛 娟 邓 鑫 罗雷雷 郭文龙 |
| 作者单位： | 1河北大学基础医学院，河北省保定市 071000；2涞水县医院急诊科，河北省涞水县074100 |
| 基金项目： | 河北省人口与计划生育委员会项目(2010-A03)；河北大学医学部预研资助项目(2007JY04)；河北大学医学部学科专项资金建设项目(2012A2002）。 |
| 摘 要： | 背景：研究显示α-玉米赤霉醇对绝经后骨质疏松症具有明显的防治作用。目的：观察α-玉米赤霉醇对体外大鼠成骨细胞骨保护素分泌及其mRNA表达的影响。方法：将体外培养的成骨细胞中分别加入雌激素和α-玉米赤霉醇(10-5~10-10 mol/L)进行干预。结果与结论：ELISA法和RT-PCR检测发现，不同浓度的α-玉米赤霉醇(10-5~10-10 mol/L)作用于成骨细胞后，骨保护素的分泌量及细胞骨保护素mRNA表达较对照组均增加(P < 0.05)。结果证实，在骨髓基质细胞来源的成骨细胞中加入α-玉米赤霉醇可促进成骨细胞骨保护素的分泌及其mRNA的表达。 |
| 关 键 词： | 成骨细胞 破骨细胞 骨保护素 &alpha -玉米赤霉醇 绝经后骨质疏松症 干细胞因子 |
| 收稿时间： | 2011-12-01 |
Effects of α-zeranol on expression of osteoprotegerin in bone marrow mesenchymal stem cells-derived osteoblasts |
|
| Liu Wei-hua,Duan Fei,Li Shao-chun,Sun Xiao-fang,Hao Hua-chao,He Jing,Xue Juan,Deng Xin,Luo Lei-lei,Guo Wen-long. Effects of α-zeranol on expression of osteoprotegerin in bone marrow mesenchymal stem cells-derived osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(6): 977-980. DOI: 10.3969/j.issn.1673-8225.2012.06.006 | |
| Authors: | Liu Wei-hua Duan Fei Li Shao-chun Sun Xiao-fang Hao Hua-chao He Jing Xue Juan Deng Xin Luo Lei-lei Guo Wen-long |
| Affiliation: | 1School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China; 2Department of Emergency, Laishui Hospital, Laishui 074100, Hebei Province, China |
| Abstract: | BACKGROUND: Studies showed that α-zeranol (α-ZAL) has significant prevention and treatment effect on postmenopausal osteoporosis.OBJECTIVE: To observe the effects of α-ZAL on the secretion of osteoprotegerin (OPG) and expression of OPG mRNA in osteoblasts of the rats. METHODS: Estrogen and α-ZAL (10-5-10-10 mol/L) were added into in vitro cultured osteoblasts respectively for intervention.RESULTS AND CONCLUSION: The results of ELISA and RT-PCR showed that after application of different concentrations of α-ZAL (10-5-10-10 mol/L), the secretion of OPG and expression of OPG mRNA in the estrogen group were higher than those in the control group (P < 0.05). The results confirmed that in the bone marrow mesenchymal stem cells-derived osteoblast, adding α-ZAL can promote osteoblast OPG secretion and mRNA expression. |
| Keywords: | |
| 点击此处可从《中国组织工程研究》浏览原始摘要信息 | |
| 点击此处可从《中国组织工程研究》下载全文 | |