超顺磁性氧化铁纳米颗粒体外标记兔骨髓间充质干细胞的安全性以及MRI成像特征*☆

背景：传统的干细胞标记方法常需要病理组织学等侵袭性手段检测，无法动态观察整个实验过程，也不适合临床研究。 目的：观察超顺磁性氧化铁纳米颗粒体外标记对兔骨髓间充质干细胞生物学特性的影响以及标记兔骨髓间充质干细胞的体外MRI成像特征。 方法：采用红细胞裂解贴壁法培养扩增兔骨髓间充质干细胞。采用不同浓度超顺磁性氧化铁纳米颗粒(100，50，25，12.5 mg/L)联合多聚赖氨酸(0.75 mg/L)标记兔骨髓间充质干细胞。对标记兔骨髓间充质干细胞进行MRI检测，观察其成像特征。 结果与结论：25 mg/L的超顺磁性氧化铁纳米颗粒联合0.75 mg/L多聚赖氨酸标记兔骨髓间充质干细胞具有较好的安全性和有效性。此浓度对细胞的生长活性没有影响，不影响骨髓间充质干细胞的成骨成脂分化能力。MRI扫描在T2*WI序列上能明显有效地显像超顺磁性氧化铁纳米颗粒标记的兔骨髓间充质干细胞。 关键词：超顺磁性氧化铁纳米颗粒；骨髓间充质干细胞；标记；磁共振；安全性 doi:10.3969/j.issn.1673-8225.2012.06.001

Security and magnetic resonance imaging features of superparamagnetic iron oxide nanoparticles labelling rabbit bone marrow mesenchymal stem cells

BACKGROUND: The traditional methods to label stem cells often need histopathology examination. It can not observe the whole experiment process dynamically and is not suitable for clinical research.  OBJECTIVE: To observe the biological characteristics of rabbit bone marrow mesenchymal stem cells (BMSCs) labeled by superparamagnetic iron oxide (SPIO) nanoparticles in vitro, and to observe the magnetic resonance imaging (MRI) features of labeled BMSCs in vitro. METHODS: Rabbit BMSCs were cultured and expanded by using red blood cells lysis and adherent in vitro. Rabbit BMSCs were labeled with SPIO of different concentrations (100, 50, 25, 12.5 mg/L) combined with polylysine (PLL) (0.75 mg/L). The MRI imaging features of labeled rabbit BMSCs were observed by MRI scanning. RESULTS AND CONCLUSION: The rabbit BMSCs labeled with 25 mg/L SPIO combined with 0.75 mg/L PLL were more efficient and safe. This level of concentration could not influence on cells activity and could not influence the osteogenic and adipogenic differentiation capacity of labeled rabbit BMSCs obviously. MRI scanning on T2 * WI sequences can show the SPIO nanoparticles labeled rabbit BMSCs significantly and effectively.