羟基脲抑制盐酸胍对于酵母朊病毒[PSI+]的治愈

摘 要：目的 研究细胞分裂与盐酸胍作用下酵母朊病毒[PSI+]聚集体解聚的关系。方法 本研究借助重组表达Sup35p-GFP的菌株，采用表型分析方法与半变性琼脂糖凝胶电泳（SDD-AGE）结合蛋白质免疫印迹技术，分析了羟基脲抑制细胞分裂情况下对于盐酸胍解聚酵母朊病毒聚集体的影响。结果 羟基脲抑制细胞分裂的情况下，表型分析的数据显示盐酸胍不能治愈酵母朊病毒[PSI+]，蛋白水平的实验数据证实了这一结果并发现，羟基脲的存在使得盐酸胍解聚酵母朊病毒聚集体的能力明显下降。结论 这暗示着盐酸胍治愈朊病毒[PSI+]是需要细胞进行分裂的。

Hydroxylurea inhibition of the curing of yeast prion [PSI +] by guanidine hydrochloride

AbstractIn order to further study the relationship between the cell division and the disaggregation of yeast prion [PSI+] with the treatment of guanidine hydrochloride, we investigated effect of hydroxylurea on guanidine hydrochloride curing yeast prion [PSI+] with a recombinant Sup35-GFP strain using the techniques of phenotype analysis and semi-denaturing agarose gelelectrophoresis (SDD-AGE) combined with western blot. Our results showed that guanidine hydrochloride was unable to cure yeast prion [PSI+] when cell division was suppressed by hydroxylurea. Further research showed that hydroxylurea could significantly decrease the ability of guanidine hydrochloride to disaggregate yeast prion [PSI+]. These results suggested that cell division is essential for the curing of yeast prion [PSI+] by guanidine hydrochloride.