HBV患者血清IL-6与T淋巴细胞亚群、HBVDNA及肝功能的关系及其临床意义

目的通过研究乙型肝炎患者体内IL-6与T淋巴细胞亚群、HBVDNA及肝功能的关系,以探讨其临床意义。方法随机选取70例乙型肝炎患者为试验组及15例健康者为对照组,上述85例均应用酶联免疫吸附（ELISA）法检测血清IL-6,流式细胞仪检测T淋巴细胞亚群。分析比较2组检测结果及相关性。结果试验各组IL-6水平显著高于对照组,与肝组织损害程度（ALT、TBiL）呈正相关;CD4^＋、CD4^＋／CD8^＋明显降低,与血清IL-6呈负相关;CD8^＋明显升高,与血清IL-6呈正相关,差异均有统计学意义（P〈0．05）;HBVDNA阳性亚组和HBVDNA阴性亚组CD4^＋、CD4^＋／CD8^＋、CD^8＋与对照组比较,差异均有统计学意义（P〈0．05）。结论乙型肝炎患者存在着细胞和细胞因子免疫功能紊乱,IL-6参与乙型肝炎免疫损害,可作为判断病情、治疗效果和评估预后的监测指标。

The relationship and clinical significance in IL-6 of serum and T-lymphocyte subsets, HBV DNA and liver function in patients with HBV

Objective To investigate the relationship between IL-6 and T-lymphocyte subsets,HBV DNA and liver faction in patients with HBV,in order to explore its clinical significance. Methods 70 cases patients with HBV were randomly joined to experimental group and 15 cases healthy peoples were added in control group. ELISA techenique was uesd to detect the level of IL-6 ; the level of T-lymphocyte subsets were detected by flow eytometry. Analyzed and compared results and corre- lation between two groups. Results The level of IL-6 in every experimental group was significantly higher than normal eohtrol group, and was correlated with liver damage level （ ALT, TBiL） （ P 〈0. 05 ） ;in the euont of CD4＋ and CD4＋/CD8 ＋ levels were significantly lower,and were negative correlation with the level of IL-6 of serum, in the euont of CDs ＋ level was significantly higher, and was correlated with the level of IL-6 of serum（P 〈 0.05 ）, compared HBV DNA（ ＋ ）group with HBV DNA（ - ） group and normal control group,the euont of CD4 ＋ cell, CD4 ＋/CD8 ＋ , CD8 ＋ cell were all significant difference （ P 〈 0.05 ）. Conclusion The cell-mediated immunity and eytokines in patients with HBV are disordered. IL-6 inwolved in the damage of hepatitis B immunization,so it can be used as illness, treatment and assessment of prognosis indicators for monitoring.