| 体外人全血热原检测方法的研究 |
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| 引用本文： | 黄清泉,刘文英,丁黎,奚廷斐,李冠民.体外人全血热原检测方法的研究[J].中国药学杂志,2004,39(5):372-374. |
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| 作者姓名： | 黄清泉 刘文英 丁黎 奚廷斐 李冠民 |
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| 作者单位： | 1. 中国药品生物制品检定所,北京,100050
2. 中国药科大学,江苏,南京,210005 |
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| 基金项目： | 科技部社会公益研究项目，北京市实验动物专项基金 |
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| 摘 要： | 目的 对体外人全血热原检测方法进行优化。方法 采用酶联免疫吸附实验(ELISA)法测定血液中释放的内源性热原。结果 TNF-α,IL-1β,IL-6这3种细胞因子均可作为测定指标;血液和供试品的反应时间确定为6 h;血液稀释倍数确定为1→2稀释;血液的保存温度确定为4℃;血液的保存时间确定为4 h;加样体积比确定为血液-供试液=4:1。结论 本研究结果为体外人全血热原检测法的标准化打下初步基础。
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| 关 键 词： | 内源性热原 热原检查 细菌内毒素 |
| 文章编号： | 1001-2494(2004)05-0372-03 |
| 收稿时间： | 2003-12-13; |
Study on in vitro pyrogen test using human whole blood |
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| HUANG Qing-quan,LIU Wen-ying,DING Li,XI Ting-fei,LI Guan-min.Study on in vitro pyrogen test using human whole blood[J].Chinese Pharmaceutical Journal,2004,39(5):372-374. |
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| Authors: | HUANG Qing-quan LIU Wen-ying DING Li XI Ting-fei LI Guan-min |
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| Institution: | 1.National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China;2.China Pharmaceutical University,Nanjing 210005,China |
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| Abstract: | OBJECTIVE To optimize the in vitro pyrogen test using human whole blood. METHODS The levels of cytokines released in blood (namely endogenous pyrogens) were detected by double antibody enzyme-linked immunoassay. RESULTS TNF-α,IL-1β and IL-6 were selected as the indicators. The optimal conditions were established as following respectively: reaction time 6 h; Blood dilution times: two; preservation temperature of blood at 4℃ and preservation time 4 h; ratios of blood to test solution in volume: 4:1. CONCLUSION The results provided basis for the standardization of the in vitro pyrogen test using human whole blood. |
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| Keywords: | endogenous pyrogen pyrogen test bacterial endotoxin cytokine |
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