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| HPLC法测定注射用夫西地酸钠的含量 | |
| 引用本文： | 王亚群,祝秀珍,陈永志. HPLC法测定注射用夫西地酸钠的含量[J]. 中国医药指南, 2008, 6(18) |
| 作者姓名： | 王亚群 祝秀珍 陈永志 |
| 作者单位： | 四川省攀枝花市中心医院,617067 |
| 摘 要： | 目的建立夫西地酸钠原料高效液相色谱法含量测定的方法。方法采用HPLC法,色谱柱为Diamonsil((钻石)C18(250×4.6 mm5μm),流动相:0.05 mol/L磷酸-乙腈-甲醇(40∶50∶10);检测波长:235nm;流速:1.0 mL/min;柱温:30℃。结果夫西地酸钠的进样量在0.379～30.35μg范围内,与其峰面积有良好的线性关系(r=0.999 9),高中低3种浓度的加样回收率为99.34%～100.66%,相对标准差(RSD)为0.17%～0.76%。结论方法简便易行、准确可靠,可用于夫西地酸钠原料的含量测定。 |
| 关 键 词： | 夫西地酸钠 HPLC 含量测定 |
Determination of sodium fusidate for injection by reversed phase high-performance liquid chromatography |
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| WANG Ya-qun,ZHU Xiu-zhen,CHEN Yong-zhi. Determination of sodium fusidate for injection by reversed phase high-performance liquid chromatography[J]. Guide of China Medicine, 2008, 6(18) | |
| Authors: | WANG Ya-qun ZHU Xiu-zhen CHEN Yong-zhi |
| Affiliation: | WANG Ya-qun; ZHU Xiu-zhen; CHEN Yong-zhi(Central Hospital of Panzhihua City; Panzhihua 617067; China); |
| Abstract: | Objective To establish the reversed phase high-performance liquid chromatography(RP-HPLC) assay for determination of the content of sodium fusidate for injection.Methods RP-HPLC assay was adopted.Diamonsil C18(250 mm×4.6 mm,5 μm) was chosen as the chromatographic column with UV detection wavelength at 235 nm,and the mobile phase consisting of 0.05mol/L phosphoric acid-acetonitrile-methanol(40∶50∶10) was used;the flow rate was 1.0 mL/min,and the column temperature was 30 ℃.Results The calibration curve was l... |
| Keywords: | HPLC |
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