再生障碍性贫血患者骨髓CD34~+和Fas~+细胞Fas抗原的表达

采用流式细胞术检测再生障碍性贫血 (AA ,以下简称再障 )患者骨髓CD34 + 细胞表面Fas抗原的表达 ,采用体外短期液体培养的方法研究干扰素γ(IFN γ)对AA患者骨髓CD34 + 细胞表面Fas抗原表达的影响 ,以探讨Fas介导的凋亡在AA发病中的作用。正常对照骨髓CD34 + 细胞表面Fas抗原的表达率为 ( 1 0 .0 2± 2 .33) % ,重型再障组(SAA)和慢性再障组 (CAA)分别为 ( 38.2 8± 9.0 1 ) %和 ( 2 6 .6 6± 4 .2 7) % ,SAA组和CAA组与对照组比较均有显著性差异 (P <0 .0 1 ,P <0 .0 5 ) ;SAA组与CAA组比较也有显著性差异 (P <0 .0 5 )。正常骨髓单个核细胞在含SCF、IL 3、GM SCF和EPO细胞因子组合的液体培养体系中培养 ,加入IFN γ时 ,CD34 + 细胞表面Fas抗原表达稍增高 ,AA患者骨髓在上述相同的条件下 ,CD34 + 细胞表面Fas抗原表达与正常对照骨髓比较显著增高 (P <0 .0 5 ) ,且SAA组明显高于CAA组 (P <0 .0 5 )。实验结果表明 ,AA患者骨髓CD34 + 细胞表面Fas的表达明显高于正常对照 ;AA患者骨髓单个核细胞体外培养时 ,IFN γ促使骨髓CD34 + 细胞表面Fas的表达作用较正常对照明显增强。结果提示 ,AA患者骨髓CD34 + 细胞表面Fas的异常表达可能与AA的发病有关。

Expression of Fas Antigen on Bone Marrow CD34+ Cells in Patients with Aplastic Anemia

The objective of the study was to investigate Fas antigen induced apoptosis in the pathogenesis of aplastic anemia. The expression of Fas antigen on bone marrow CD34⁺ cells and the effect of IFN-γ to it were assayed in AA by flowing cytometry and short-liquid-culture in vitro. Fas antigen expression on CD34⁺ cells in SAA and CAA was significantly higher than that in normal controls 〔(38.28± 9.01)% and(26.66±4.27)% vs(10.02±2.33)%, (P<0.01, (P<0.05 respectly〕. Fas antigen expression on CD34⁺ cells in CAA was significantly lower than that in SAA(P<0.05). Addition of IFN-γ to liquid culture of BMMNC including SCF, IL-3, GM-CSF and EPO cytokines showed significant stimulating effects on Fas antigen expression in AA, but not in normal controls, the expression in SAA was higher than that in CAA(P<0.05). The abnormal expression of Fas antigen on CD34⁺ cells may be involved in the pathogenesis of AA.