Role of Acinetobacter baumannii UmuD Homologs in Antibiotic Resistance Acquired through DNA Damage-Induced Mutagenesis |
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| Authors: | Jesús Aranda Mario López Enoy Leiva Andrés Magán Ben Adler Germán Bou Jordi Barbé |
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| Affiliation: | aDepartament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona (UAB), Barcelona, Spain ;bServizo de Microbioloxía-INIBIC, Complexo Hospitalario Universitario A Coruña (CHUAC), A Coruña, Spain ;cAustralian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Clayton, Victoria, Australia |
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| Abstract: | ![]()
The role of Acinetobacter baumannii ATCC 17978 UmuDC homologs A1S_0636-A1S_0637, A1S_1174-A1S_1173, and A1S_1389 (UmuDAb) in antibiotic resistance acquired through UV-induced mutagenesis was evaluated. Neither the growth rate nor the UV-related survival of any of the three mutants was significantly different from that of the wild-type parental strain. However, all mutants, and especially the umuDAb mutant, were less able to acquire resistance to rifampin and streptomycin through the activities of their error-prone DNA polymerases. Furthermore, in the A. baumannii mutant defective in the umuDAb gene, the spectrum of mutations included a dramatic reduction in the frequency of transition mutations, the mutagenic signature of the DNA polymerase V encoded by umuDC. |
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