| 氧化苦参碱对大鼠肝星状细胞增殖的影响 |
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| 引用本文： | 卢清 张清波 邬祥惠 谭英姿 刘成海. 氧化苦参碱对大鼠肝星状细胞增殖的影响[J]. 肝脏, 2001, 6(1): 17-18 |
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| 作者姓名： | 卢清 张清波 邬祥惠 谭英姿 刘成海 |
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| 作者单位： | 卢清（200040,复旦大学医学院华山医院传染科）;张清波（200040,复旦大学医学院华山医院传染科）;邬祥惠（200040,复旦大学医学院华山医院传染科）;谭英姿（上海中医药大学肝病研究所）;刘成海（上海中医药大学肝病研究所） |
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| 摘 要： | 目的探讨氧化苦参碱对大鼠肝星状细胞增殖的影响及其抗肝纤维化的机理.方法用链酶蛋白酶和胶原酶原位灌流消化正常大鼠肝脏,Nycodenz密度梯度离心分离肝星状细胞,以MTT比色法观察氧化苦参碱对肝星状细胞毒性作用,3H-TdR法观察氧化苦参碱对肝星状细胞增殖的效应.结果氧化苦参碱浓度＞10-5mol/1时对肝星状细胞增殖有抑制作用(P＜0.05).结论氧化苦参碱可抑制肝星状细胞的增殖,有抗肝纤维化的作用.
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| 关 键 词： | 肝星状细胞 氧化苦参碱 细胞增殖 大鼠 肝纤维化 |
| 修稿时间： | 2000-06-29 |
Effect of oxymatrine on proliferation of hepatic stellate cells of rat liver |
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| LU Qing,ZHANG Qingbo,WU Xianghui,et al.. Effect of oxymatrine on proliferation of hepatic stellate cells of rat liver[J]. Chinese Hepatology, 2001, 6(1): 17-18 |
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| Authors: | LU Qing ZHANG Qingbo WU Xianghui et al. |
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| Affiliation: | LU Qing,ZHANG Qingbo,WU Xianghui,et al. Department of Infectious Diseases,Huashan Hospital,Medical School of Fudan University,Shanghai 200040 |
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| Abstract: | Objective To investigate the effect of oxymatrine on proliferation of hepatic stellate cells (HSC) of rat liver.Methods HSCs were isolated and cultured from the liver of normal rat by in situ perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz. MTT colorimetry was used for assaying cytotoxicity and incorporation of 3H TdR for assaying proliferation of HSC. Results >10 -5 mol/l oxymatrine can inhibit HSC proliferation ( P <0.05). Conclusion Oxymatrine may prevent hepatic fibrosis by inhibiting HSC proliferation. |
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| Keywords: | Hepatic stellate cell Oxymatrine Proliferation |
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