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| 丙酸氟替卡松局部治疗鼻息肉作用机制的探讨 | |
| 引用本文： | 孙臻峰,陈霞芳,张渊. 丙酸氟替卡松局部治疗鼻息肉作用机制的探讨[J]. 中国临床药学杂志, 2008, 17(6) |
| 作者姓名： | 孙臻峰 陈霞芳 张渊 |
| 作者单位： | 1. 上海交通大学附属第一人民医院,耳鼻喉科,上海,200080 2. 上海交通大学附属第一人民医院,中心实验室,上海,200080 3. 上海交通大学附属第一人民医院,药剂科,上海,200080 |
| 基金项目： | 上海市第一人民医院基础研究类院级课题 |
| 摘 要： | 目的 观察丙酸氟替卡松对体外培养的鼻息肉成纤维细胞生长及凋亡的影响,以探讨其局部治疗鼻息肉的可能机制.方法 取11例鼻息肉患者的鼻息肉组织进行传代培养后获得鼻息肉成纤维细胞,观察在不同体积分数(2%、4%、8%)丙酸氟替卡松作用铝、72、96和120 h对成纤维细胞活力的影响;采用流式细胞仪和Hoechest染色观察丙酸氟替卡松(体积分数为4%)作用48、72、96和120 h时对成纤维细胞凋亡的影响,并与空白对照组进行对比.结果 与空白对照组相比,当丙酸氟替卡松的体积分数>4%时能显著抑制成纤维细胞的生长(P<0.05).流式细胞仪和Hoechest染色结果表明,体积分数为4%的丙酸氟替卡松能导致成纤维细胞凋亡.与空白对照组对比差异有统计学意义(P<0.05).结论 丙酸氟替卡松体外能抑制鼻息肉成纤维细胞的生长并诱导其凋亡. |
| 关 键 词： | 丙酸氟替卡松 成纤维细胞 鼻息肉 凋亡 |
Study of mechanism of nasal polyps treatment with fluticasone propionate |
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| SUN Zhenfeng,CHEN Xiafang,ZHANG Yuan. Study of mechanism of nasal polyps treatment with fluticasone propionate[J]. Chinese Journal of Clinical Pharmacy, 2008, 17(6) | |
| Authors: | SUN Zhenfeng CHEN Xiafang ZHANG Yuan |
| Affiliation: | SUN Zhenfeng1,CHEN Xiafang2,ZHANG Yuan3(1Department of Otolaryngology,2Central Laboratory,3Department of Pharmacy,The First People's Hospital,Shanghai Jiaotong University,Shanghai 200080,China) |
| Abstract: | AIM To observe the effect of fluticasone propionate(FP)on apoptosis in nasal polyps fibroblast.METHODS Nasal polyps fibroblasts derived from 11 nasal polyps were cultured with different concentrations(2%,4%,8%)of FP.The growth of nasal polyps fibroblasts were observed after 48,72,96 and 120 h.Subsequently,the ability of FP induced cell apoptotic of fibroblasts was examined by flow cytometric meter and dye of Hoechest.RESULTS Addition of FP at a concentration of >4% to cell cultures inhibited cell growth in ... |
| Keywords: | fluticasone propionate fibroblast nasal polyp apoptosis |
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