| 实验性哮喘小鼠免疫细胞的ADCC作用 |
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| 引用本文： | 袁继红,董泽华,黄瑾. 实验性哮喘小鼠免疫细胞的ADCC作用[J]. 吉林大学学报(医学版), 2003, 29(1): 67-68 |
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| 作者姓名： | 袁继红 董泽华 黄瑾 |
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| 作者单位： | 吉林大学第二医院检验科,吉林,长春,130041;吉林大学第二医院呼吸内科,吉林,长春,130041 |
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| 摘 要： | 目的 :探讨支气管哮喘的发病机制。方法 :在小鼠 OVA支气管哮喘模型的基础上 ,分离哮喘鼠和正常鼠脾脏各种免疫细胞 ,包括单核 /巨噬细胞、树突状细胞、B细胞、CD3+ T细胞、CD4+ T细胞和 CD8+ T细胞 ,并以细胞增殖法和改良的 MTT比色法检测上述各种细胞的抗体依赖的细胞介导细胞毒效应 ( antibody dependentcell- mediated cytotoxicity,ADCC)。结果 :支气管哮喘小鼠上述各种细胞自然状态下的 ADCC作用均较健康对照组减弱 ( P<0 .0 5 )。结论 :免疫细胞自然状态下 ADCC作用减弱可能作为支气管哮喘免疫功能紊乱的一部分参与了哮喘的发病
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| 关 键 词： | 哮喘/病因学 免疫细胞 抗体依赖的细胞介导 细胞毒作用 疾病模型 动物 细胞毒性 免疫 |
| 文章编号： | 1671-587X(2003)01-0067-02 |
| 修稿时间： | 2002-03-12 |
ADCC Effect of Immunocytes in Experimental Mouse with Asthma |
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| YUAN Ji hong ,DONG Ze hua ,HUANG Jin. ADCC Effect of Immunocytes in Experimental Mouse with Asthma[J]. Journal of Jilin University: Med Ed, 2003, 29(1): 67-68 |
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| Authors: | YUAN Ji hong DONG Ze hua HUANG Jin |
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| Affiliation: | YUAN Ji hong 1,DONG Ze hua 2,HUANG Jin 3 |
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| Abstract: | Objective:To investigate the mechanism of bronchial asthma. Methods:An experimental asthmatic mouse model with OVA was established. Then immunocytes including monocytes/macrophages, dendritic cells, B cells, CD3 +T cells, CD4 +T cells and CD8 +T cells were isolated from the mice of asthma group and controlgroup, respectively. Methods: Cell proliferation and improved MTT Colorimetry were used to detect ADCC effect of all those cells. Results:The ADCC effects of those natural immunocytes were weaker in mice with asthma than health control, P <0.05. Conclusion:The abnormality of ADCC effect of immunocytes may be an important part of it′s immunological disorder and be one of the mechanisms in pathogensis of asthma. |
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| Keywords: | asthma/etiology immunocyte antibody dependent cell mediated cytotoxicity (ADCC) disease models animal cytotoxicity immunologic |
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