| 食品中污染酵母菌的RAPD分类鉴定 |
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| 引用本文： | 何培彦.食品中污染酵母菌的RAPD分类鉴定[J].河南预防医学杂志,2008,19(1):6-7. |
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| 作者姓名： | 何培彦 |
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| 作者单位： | 嘉兴市疾病预防控制中心,浙江,嘉兴,314050 |
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| 摘 要： | 目的探索采用RAPD技术对食品污染酵母进行分类鉴定的可行性。方法采用RAPD技术对从食品中分离到的22株污染酵母菌进行分子分型并以UPGMA法构建以上菌株的带型关系系统树状图。结果22株污染酵母菌总共扩增出11种不同的指纹图谱，经聚类分析可分为2个聚类群。结论RAPD技术可以作为一种有效、快速、简便的对污染酵母菌进行分类鉴定的手段。
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| 关 键 词： | 酵母 RAPD 分类鉴定 |
| 文章编号： | 1006-8414（2008）01-0006-02 |
| 收稿时间： | 2007-10-05 |
| 修稿时间： | 2007年10月5日 |
Identification of food spoilage yeast by RAPD technique |
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| HE Pei-yan.Identification of food spoilage yeast by RAPD technique[J].Henan Journal of Preventive Medicine,2008,19(1):6-7. |
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| Authors: | HE Pei-yan |
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| Institution: | HE Pei -yan.( Jiaxing Center for Diseases Control and Prevention, Zhejiang Jiaxing 314050 , China ) |
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| Abstract: | Objective Exploring the feasibility of RAPD technique for identification of food spoilage yeast.MethodAdopting RAPD technique to conduct molecular typing of 22 strains of food spoilage yeast and constructing the dendrogram by UPGMA method.Result11 DNA fingerprints were obtained and 22 spoilage yeast could be classified into two groups.ConclusionRAPD technique was a effective,fast and simple method for identification of food spoilage yeast. |
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| Keywords: | Yeast RAPD Identification |
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