| 小鼠共刺激分子B7-1的分子克隆、测序与真核表达载体的构建 |
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| 引用本文： | 周保国,乔海泉,代文杰,潘尚哈,姜洪池. 小鼠共刺激分子B7-1的分子克隆、测序与真核表达载体的构建[J]. 哈尔滨医科大学学报, 2002, 36(6): 428-430 |
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| 作者姓名： | 周保国 乔海泉 代文杰 潘尚哈 姜洪池 |
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| 作者单位： | 哈尔滨医科大学第一临床医学院普外科,哈尔滨医科大学肝脾外科实验中心,黑龙江,哈尔滨,150001;哈尔滨医科大学第一临床医学院普外科,哈尔滨医科大学肝脾外科实验中心,黑龙江,哈尔滨,150001;哈尔滨医科大学第一临床医学院普外科,哈尔滨医科大学肝脾外科实验中心,黑龙江,哈尔滨,150001;哈尔滨医科大学第一临床医学院普外科,哈尔滨医科大学肝脾外科实验中心,黑龙江,哈尔滨,150001;哈尔滨医科大学第一临床医学院普外科,哈尔滨医科大学肝脾外科实验中心,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金资助项目(30170916,30070739),黑龙江省自然科学基金资助项目(D01-04) |
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| 摘 要： | 目的：克隆小鼠共刺激分子B7－1基因,测序并构建其真核表达载体。方法：从小鼠脾组织中提取总RNA,用RT－PCR方法将B7－1的DNA扩增出,克隆到真核表达载体pcDNA3中,测定其cDNA序列。结果：测序结果表明我们克隆的B7－1基因序列与GenBank中序列有1个碱基不同,所编码的氨基酸发生突变。结论：已成功构建小鼠B7－1的真核表达载体,为进一步应用B7－1进行肿瘤基因治疗打下基础。
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| 关 键 词： | B7-1 克隆 载体 序列 |
| 文章编号： | 1000-1905(2002)06-0428-03 |
| 修稿时间： | 2002-06-17 |
Cloning, sequencing and construction of eukaryotic expression vector for B7-1 gene |
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| ZHOU Bao-guo,QIAO Hai-quan,DAI Wen-jie,et al. Cloning, sequencing and construction of eukaryotic expression vector for B7-1 gene[J]. Journal of Harbin Medical University, 2002, 36(6): 428-430 |
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| Authors: | ZHOU Bao-guo QIAO Hai-quan DAI Wen-jie et al |
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| Abstract: | Objective To clone, sequence and construct eukaryotic expression vector of mouse B7-1 gene. Methods The total RNA was isolated from mouse spleen.The B7-1 cDNA was synthesized and amplied from the mRNA by RT-PCR and the PCR product was cloned into pcDNAS eukaryotic expression vector and subjected to DNA sequencing. Results Sequencing analysis revealed that the amino acid sequence of mouse B7-1 had one variation in nucleotide sequence which caused one amino acid residue displace( compared with that of published sequence). Conclusion We successfully construct the eukaryotic expression vector of mouse B7-1 gene, which provide a solid basis for the study of B7-1 on antitumor genetherapy. |
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| Keywords: | B7-1 clone vector sequence |
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