| 八珍汤对荷结肠癌小鼠皮下移植瘤中CXCR4和CD133共表达的影响 |
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| 引用本文： | 魏海梁,李京涛,闫曙光,郭辉,司明明,肖刚,陈志国.八珍汤对荷结肠癌小鼠皮下移植瘤中CXCR4和CD133共表达的影响[J].现代中西医结合杂志,2019(21):2297-2300,2304. |
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| 作者姓名： | 魏海梁 李京涛 闫曙光 郭辉 司明明 肖刚 陈志国 |
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| 作者单位： | 陕西中医药大学附属医院;陕西中医药大学 |
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| 基金项目： | 陕西省科技厅社会发展公关项目(2017SF-338;2018SF-284);陕西省中医药管理局科研计划项目(JCMS042);咸阳市科技局资助项目(2017K02-84) |
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| 摘 要： | 目的观察八珍汤对结肠癌皮下移植瘤模型小鼠癌组织中CXCR4及CD133共表达的影响,探讨八珍汤抑制结肠癌移植瘤生长的机制。方法将70只裸鼠随机分为模型组35只、八珍汤组35只。2组均采用结肠癌CT26细胞混悬液直接注射的方法建立皮下移植瘤模型,造模后第10天起,模型组以无菌蒸馏水灌胃,八珍汤组以八珍汤灌胃,均连续灌胃10d。自灌胃开始后第2,4,6,8,10天测量肿瘤大小并计算体积,然后计算抑瘤率。灌胃后的第11天处死2组裸鼠,取瘤组织,常规HE染色观察,并采用免疫组化法检测瘤组织中CXCR4和CD133表达情况。结果灌胃后第2,4,6,8,10天,八珍汤组肿瘤体积均明显小于模型组(P均<0.05),八珍汤组抑瘤率为56.13%。移植瘤组织HE染色显示,八珍汤组较模型组细胞异型性小,且细胞排列成团现象不明显。2组CXCR4、CD133蛋白阳性表达率比较虽然差异均无统计学意义(P均>0.05),但八珍汤组约较模型组降低10%。结论八珍汤可以通过抑制CXCR4及CD133的共表达来抑制肿瘤的生长。
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| 关 键 词： | CXCR4 CD133 八珍汤 结肠癌移植瘤 荷瘤小鼠 |
Effect of Bazhen Decoction on co-expression of CXCR4 and CD133 in subcutaneously transplanted tumor of mice with colon cancer |
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| WEI Hailiang,LI Jingtao,YAN Shuguang,GUO Hui,SI Mingming,XIAO Gang,CHEN Zhiguo.Effect of Bazhen Decoction on co-expression of CXCR4 and CD133 in subcutaneously transplanted tumor of mice with colon cancer[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2019(21):2297-2300,2304. |
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| Authors: | WEI Hailiang LI Jingtao YAN Shuguang GUO Hui SI Mingming XIAO Gang CHEN Zhiguo |
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| Institution: | (Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,Shaanxi,China;Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,Shaanxi,China) |
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| Abstract: | WEI Hailiang;LI Jingtao;YAN Shuguang;GUO Hui;SI Mingming;XIAO Gang;CHEN Zhiguo(Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,Shaanxi,China;Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,Shaanxi,China) |
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| Keywords: | CXCR4 CD133 Bazhen decoction colon cancer xenograft tumor-bearing mice |
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