| RP-HPLC法测定天王补心丸中梓醇的含量 |
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| 引用本文： | 杨仕林,李小刚,谢云.RP-HPLC法测定天王补心丸中梓醇的含量[J].中国药房,2012(24):2289-2290. |
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| 作者姓名： | 杨仕林 李小刚 谢云 |
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| 作者单位： | [1]湖北阳新县人民医院,湖北阳新435200 [2]湖北中医药大学,武汉430064 |
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| 摘 要： | 目的:建立天王补心丸中梓醇含量的测定方法。方法:采用反相高效液相色谱法。色谱柱为Agilent-ZORBX SB-C18柱,流动相为乙腈-0.1%磷酸溶液(V/V)=1:99,流速为1.0mL.min-1,检测波长为210nm,进样量为20μL,柱温为40℃。结果:梓醇检测浓度在2.004～20.046μg.mL-1范围内与其峰面积积分值呈良好的线性关系(r=0.9993);平均加样回收率为99.9%,RSD=2.2%(n=9)。结论:该法简便、准确、重复性好,适用于天王补心丸的质量控制。
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| 关 键 词： | 天王补心丸 梓醇 含量测定 反相高效液相色谱法 |
Determination of Catalpol in Tianwang Buxin Pill by RP-HPLC |
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| YANG Shi-lin,LI Xiao-gangHubei Province Yangxin County People＇s Hospital,Hubei Yangxin,China XIE Yun.Determination of Catalpol in Tianwang Buxin Pill by RP-HPLC[J].China Pharmacy,2012(24):2289-2290. |
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| Authors: | YANG Shi-lin LI Xiao-gangHubei Province Yangxin County People＇s Hospital Hubei Yangxin China XIE Yun |
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| Institution: | YANG Shi-lin, LI Xiao-gang(Hubei Province Yangxin County People＇s Hospital, Hubei Yangxin 435200, China) XIE Yun(Hubei University of Chinese Medicine, Wuhan 430064, China) |
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| Abstract: | OBJECTIVE： To establish a method for determination of catalpol in Tianwang buxin pill. METHODS： The HPLC systerm consisted of Agilent-ZORBX SB-C18 column, acetonitrile-0.1% phosphoric acid（ V/V= 1：99） solution was used as the mobile phase, the detector wavelength was set at 210 nm and column temperature was 40 ℃, the flow rate was 1.0 mL.min^-1. In- jection volume was 20μL. RESULTS： The linear range of catalpol was 2.004-20.046 μg.mL^-1 （r=0.999 3）. The average recovery was 99.9%, RSD was 2.2% （n=9）. CONCLUSION： The method is simple, accurate and reproducible, which can be used for determination of catalpol in Tianwang buxin pill. |
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| Keywords: | Tianwang buxin pill Catalpol Content determination RP-HPLC |
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