| RP-HPLC法测定妇炎康片中丹参素的含量 |
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| 引用本文： | 杨仕林,王雨来.RP-HPLC法测定妇炎康片中丹参素的含量[J].中国药房,2012(20):1909-1910. |
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| 作者姓名： | 杨仕林 王雨来 |
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| 作者单位： | [1]湖北阳新县人民医院,湖北阳新435200 [2]黄石市中心医院,湖北黄石435000 |
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| 摘 要： | 目的:建立测定妇炎康片中丹参素含量的方法。方法:采用反相高效液相色谱法。色谱柱为Lichrospher C18柱,流动相为甲醇-0.05%磷酸溶液(梯度洗脱),流速为1.0mL·min-1,检测波长为280nm,进样量为10μL,柱温为40℃。结果:丹参素进样量在0.21～0.84μg范围内与其峰面积积分值呈良好的线性关系(r=0.9996),平均回收率为98.4%,RSD=2.1%(n=9)。结论:本法简便、准确、重复性好,可用于妇炎康片的质量控制。
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| 关 键 词： | 妇炎康片 丹参素 含量测定 反相高效液相色谱法 |
Content Determination of Danshensu in Fuyankang Tablets by RP-HPLC |
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| YANG Shi-lin,WANG Yu-lai.Content Determination of Danshensu in Fuyankang Tablets by RP-HPLC[J].China Pharmacy,2012(20):1909-1910. |
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| Authors: | YANG Shi-lin WANG Yu-lai |
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| Institution: | (Hubei Province Yangxin County People＇s Hospital, Hubei Yangxin 435200, China; Huangshi Central Hospital, Hubei Huangshi 435000, China) |
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| Abstract: | OBJECTIVE： To establish the method for the content determination of danshensu in Fuyankang tablets. METH- ODS： RP-HPLC method was adopted. The determination was performed on Lichrospher C18 column with methanol-0.05% phosphoric acid solution as mobile phase （gradient elution） at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 280 nm, sample size was 10 μL. and column temperature was 40 ℃. RESULTS： The linear range of danshensu was 0.21-0.84 μg （r= 0.999 6） with an average recovery of 98.4 % （RSD = 2.1%, n = 9）. CONCLUSION： The method is simple, accurate and reproduc- ible, which can be used for the content determination of danshensu in Fuyankang tablets. |
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| Keywords: | Fuyankang tablets Danshensu Content determination RP-HPLC |
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