| 高效液相色谱法测定血清中海洛因 |
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| 引用本文： | 阳波 王佐. 高效液相色谱法测定血清中海洛因[J]. 中国药房, 1999, 10(6): 266-267 |
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| 作者姓名： | 阳波 王佐 |
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| 作者单位： | 衡阳医学院附属第一医院药剂科!衡阳市421001(阳波)，衡阳医学院心血管病研究所!衡阳市421001(王佐，万载阳) |
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| 摘 要： | 目的 :采用高效液相色谱法测定人体血清中海洛因的浓度。方法 :用反相C18 柱 ,0 12 %三氟乙酸水溶液 (A)和100 %甲醇 (B)为流动相 ,B :0 %～80 % ,6min ,流速 :1ml/min ,进行梯度洗脱 ,285nm检测。结果 :海洛因的线性范围为1 56～400ng/μl,γ=0 9997 ,最低检测浓度为0 015ng/μl,平均回收率108 45 % ,RSD为7 29 % (n=5)。结论 :本法操作简单、快速、准确
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| 关 键 词： | 高效液相色谱法 海洛因 血清浓度 |
Determination of Heroin in Serum by HPLC |
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| YANG Bo. Determination of Heroin in Serum by HPLC[J]. China Pharmacy, 1999, 10(6): 266-267 |
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| Authors: | YANG Bo |
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| Abstract: | OBJECTIVE:To study the use of high performance liquid chromatography(HPLC)method to determine heroin in serum.METHODS:The reverse C18 column was used.The mobile phase was 0 12%TFA(A)and 100% methanol(B),B:0%～80%,6 min,the flow rate is 1ml/min,after grade wash out,detecting at 285nm.RESULTS:The linear range was from 1 56 to 400 ng/μl,γ=0 9 997; The lowest concentration of detection was 0 015 ng/μl.The average recovery was 108 45%,RSD was 7 29%(n=5).CONCLUSION:This method is not only sensitive and rapid but also easy to operate. |
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| Keywords: | HPLC heroin serum concentration |
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