缺血后处理对大鼠移植胰腺细胞凋亡的影响

目的探讨缺血后处理(IPO)对大鼠移植胰腺细胞凋亡的影响及其机制。方法取12只糖尿病SD大鼠随机分为缺血再灌注组(I/R组,n=6)和缺血后处理组(IPO组,n=6),I/R组和IPO组均行胰腺移植。另取12只健康SD大鼠为供体、6只糖尿病SD大鼠为假手术组(对照组);检测各组再灌注前、后血糖;TUNEL法观察移植胰腺组织细胞凋亡情况,W estern B lot检测移植胰腺组织Bax和B cl-2蛋白表达。结果 再灌注后IPO组较I/R组血糖低(P<0.01),移植胰组织中A I值低(P<0.01),IPO组再灌注后移植胰组织Bax蛋白低表达,B cl-2蛋白高表达。结论缺血后处理可以减少移植胰腺缺血再灌注后的细胞凋亡,机制可能与上调B cl-2蛋白和下调Bax蛋白有关。

Effects of ischemic postconditioning on graft apoptosis after pancreas transplantation in rats

Objective To evaluate the effects of ischemic postconditioning(IPO) on apoptosis of the graft after pancreas transplantation in rats,and to analyze the possible mechanism.Methods Group Sham which consist 6 normal SD rats underwent sham operation.And 12 steptozozin-induced diabetic SD rats were randomly assigned to 2 groups: Group I/R(ischemic reperfusion) consisting of 6 diabetic rats which received pancreas transplantation normally,without additional intervention,and Group IPO(ischemic postconditioning) consisting of 6 diabetic rats which received pancreas transplantation and postconditioning with 30-second reperfusion followed by 30-second reocclusion for thrice at the onset of reperfusion after cold ischemia.The blood glucose,the apoptotic cells(TUNEL),the expression of Bcl-2 and Bax protein(Western Blot) in the graft were monitored at 2 hours after long-time reperfusion.Results(1) The mean blood glucose level was lower in Group IPO than in Group I/R(P<0.05).(2) The apoptotic index in Group IPO was lower than that in Group I/R(P<0.05),the expression of the Bax protein in Group I/R was higher than that of Group IPO,and the expression of the Bcl-2 protein in Group IPO was higher than that in Group I/R.Conclusion Ischemic postconditioning can reduce apoptosis of the graft,for it may regulate the expressions of Bcl-2 and Bax protein.