大肠癌组织p16基因甲基化与临床病理特征的关系

目的：研究p16基因启动子CpG区甲基化的改变与大肠癌发生及其临床病理特征的关系。方法：采用甲基化特异PCR技术（MSP法）研究大肠癌组织p16基因甲基化，并分析其与临床病理特征的关系。结果：30例大肠癌组织中有12例（40．0％）呈p16CpG区甲基化阳性。DukesC、D期组织p16CpG区甲基化发生率明显高于DukesA、B期。有转移的大肠癌组织p16甲基化发生率（84．6％）明显高于无转移者（5．9％）。结论p16启动子区甲基化导致p16抑癌基因失活，参与大肠癌的发生和发展。大肠癌组织p16CpG区甲基化与Dukes分期、肿瘤转移有关。p16甲基化可作为评估大肠癌患者预后的1个指标。

p16 CpG Region Methylation of the Colorectal Cancer and its Association withClinicopathological Characteristics

Objective To explore whether methylation of the CpG island in the promotor of the p16 tumor suppressor gene is associated with development and clinicopathological characteristics of the colorectal cancer.Methods Methylation-specific PCR(MSP) was used to detect p16 methylation of the colorectal cancer specimen.Results In 30 sporadic colorectal cancer,40.0% had detectable p16 methylation.The patients with Dukes C,D were more likely to contain methylated p16 compared with those with Dukes A,B.The patients with tumor metastasis were more likely to contain methylated p16(84.6%) compared with those without tumor metastasis(5.9%).Conclusion p16 methylation plays a role in the carcinogenesis of colorectal cancer.Dukes stage and tumor metastasis is associated with p16 methylation status.p16 methylation might be considered as a prognostic indicator.