| 高效分子排阻色谱法测定头孢米诺钠聚合物的含量 |
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| 引用本文： | 李兵,肖甜甜. 高效分子排阻色谱法测定头孢米诺钠聚合物的含量[J]. 中国医药指南, 2013, 0(26): 344-345 |
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| 作者姓名： | 李兵 肖甜甜 |
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| 作者单位： | 吉林省食品药品检验所,吉林长春130033 |
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| 摘 要： | 目的建立高效分子排阻色谱（HPSEC）法测定头孢米诺钠聚合物。方法采用高效凝胶色谱柱；流动相为pH7．0的0．005mol／L的磷酸盐缓冲液[0．005mol／L的磷酸氢二钠：0．005mol／L的磷酸二氢钠（61：39）]；流速0．5mL／min；检测波长254nm；进样量为10μL；自身对照外标法定量。结果样品测定的线性范围为0．4-2．5mg／mL（R=0．9995）。结论该方法能够较好地分离头孢米诺钠和聚合物，简便快速，定量准确，重现性好，可用于头孢米诺钠中聚合物的检验。
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| 关 键 词： | 头孢米诺钠 头孢米诺钠聚合物 高效分子排阻色谱法 TSKG2500PWxL柱 |
Determination of the Polymers in Cefiaainox Sodium by HPSEC |
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| LI Bing,XIAO Tian-tian. Determination of the Polymers in Cefiaainox Sodium by HPSEC[J]. Guide of China Medicine, 2013, 0(26): 344-345 |
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| Authors: | LI Bing XIAO Tian-tian |
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| Affiliation: | (Jilin Institute for Food and Drugs Control, Changchun 130033, China) |
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| Abstract: | Objective To establish a high molecular exclusion chromatography （HPSEC） method for the determination of sodium cefminox polymer. Methods The efficient gel column; mobile phase （pH 7.0） 0.005mol/L phosphate buffer solution [0.005mol/L disodium hydrogen phosphate： 0.005mol/L sodium dihydrogen phosphate （61 ： 39）]; flow rate of 0.5mL/min; detection wavelength 254 nm; injection volume was 10μl; self-control external standard. Results The linear range of the sample measured 0.4-2.5mg/mL （R=0.9995）. Conelusion This method can be well separated Cefminox sodium and polymers, simple, rapid, accurate, reproducible, and can be used in the polymer Cefminox sodium test. |
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| Keywords: | Cefrainox sodium Cefminox sodium polymer Efficient molecular exclusion chromatography TSK G2500 PWxL column |
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