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| 二脱氧肌苷引发培养的背根神经节神经元的形态学改变 | |
| 引用本文： | 刘花香,刘真,杨向东,王丽红,李振中. 二脱氧肌苷引发培养的背根神经节神经元的形态学改变[J]. 神经解剖学杂志, 2008, 24(1): 8-12 |
| 作者姓名： | 刘花香 刘真 杨向东 王丽红 李振中 |
| 作者单位： | 山东大学齐鲁医院,风湿内科,济南250012;山东大学医学院,解剖学教研室,济南250012;山东大学齐鲁医院,肾内科,济南250012 |
| 基金项目： | 教育部留学回国人员科研启动基金 , 山东省优秀中青年科学家研究奖励基金 , 山东省自然科学基金 |
| 摘 要： | 为探讨二脱氧肌苷(ddI)对培养的背根神经节(DRG)神经元的影响,我们对分散培养的胎鼠DRG神经元培养3d后,再分别以不同浓度的ddI(1μg/ml,5μg/ml,10μg/mll和20μg/ml)孵育3d。终止培养后,行微管相关蛋白2(MAP2)标记,用共聚焦激光扫描显微镜观察神经元胞体和突起的改变。结果表明,DRG神经元用ddI孵育3d,神经元突起的数目减少和长度变短,呈剂量依赖性,而神经元的直径则没有变化。本研究的结果表明,ddI可影响培养的DRG神经元突起的再生和生长。 |
| 关 键 词： | 二脱氧肌苷 背根神经节 神经元 大鼠 |
| 收稿时间： | 2007-03-29 |
| 修稿时间： | 2007-03-29 |
Morphological alterations induced by didanosine in cultured dorsal root ganglion nrurons |
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| Liu Huaxiang,Liu Zhen,Yang Xiangdong,Wang Lihong,Li Zhenzhong. Morphological alterations induced by didanosine in cultured dorsal root ganglion nrurons[J]. Chinese Journal of Neuroanatomy, 2008, 24(1): 8-12 | |
| Authors: | Liu Huaxiang Liu Zhen Yang Xiangdong Wang Lihong Li Zhenzhong |
| Abstract: | To investigate the effects of didannsine(ddI)on the morphological alterations of dorsal root ganglion(DRG)neurons,dissoci-ated DRG cells from rat embryo were studied.DRG cells were cultured for 3 days and then treated with ddI for additional 3 claysin differ-ent concentrations(1μg/ml,5 μg/ml,10μg/ml and 20 μg/ml,respectively).Afarthat,DRG cells were processedformicrotubule as-soeiated protein 2(MAP2)labeling and observed under confocal laser scanning microscopy(CLSM).The results showed that both thenumber and length of neurites of the DRG cells after exposed to ddl significantly down-regulated in a dose-dependentmanner compared withcontrol group,thus suggesting that ddI may have inhibitory effects on neufite regeneration and outgrowth in dissociated DRG cultures. |
| Keywords: | didanosine dorsal root ganglion neuron rat |
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