不同浓度葡萄糖对人成骨肉瘤细胞株MG63活性的影响

目的观察不同浓度葡萄糖对体外培养的人成骨肉瘤细胞株MG63的影响,探讨糖尿病导致骨质疏松症可能的发病机制。方法采用4种不同浓度葡萄糖(5.5 mmol/L、10 mmol/L、20 mmol/L、40 mmol/L),培养基连续培养人成骨肉瘤细胞株MG63,观察葡萄糖对细胞增殖、分泌活性产物的能力、凋亡以及矿化能力等各个方面的影响。结果升高的葡萄糖浓度对细胞的增殖具有一定的促进作用,但增多的细胞数量并没有带来相应细胞活性的增加。相反,细胞分泌碱性磷酸酶(ALP)和骨钙素(BGP)的能力以及矿化能力不同程度的出现了下降,且在不考虑渗透压因素情况下,细胞凋亡比率增加。结论本实验证实了高糖环境对细胞活性,特别是矿化能力的抑制作用,为临床工作中严格控制血糖防止骨质疏松症的发生提供了理论依据。

The Influence of Different Glucose Concentrations on Cellular Activity of Human Osteosarcoma Cell Line MG63

Objective In this research,we took an insight into the influence of different glucose concentrations on human osteosarcoma cell line MG63,hoping to give an explanation to the mechanism of the osteoporosis(OP) caused by diabetes mellitus(DM).Methods Four culture media of different glucose concentrations,5.5 mmol/L,10 mmol/L,20 mmol/L,40 mmol/L,were set up for this study.Human osteosarcoma cell line MG63 was cultured in these media successively for 21 d,and then the proliferation rate of cells,secretion of ALP and BGP,percentage of apoptosis cells and the formation of calcified nods were observed and measured.Results The elevated glucose concentration prompted the proliferation of MG63 cells,but the activities of MG63 cells were not correspondent with the cell number.On the contrary,the secretion of ALP,BGP and the mineralization of the MG63 cells were decreased.The apoptosis of the cells was promoted.Conclusion From our study,we confirmed the toxicity of hyperglycemia to MG63 cells.It suggested that the tight control of the serum glucose can be a good method to prevent the occurrence of osteoporosis.