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| HPLC法同时测定复方硝酸咪康唑乳凝胶中硝酸咪康唑及蛇床子素的含量 | |
| 引用本文： | 陈艳玲,刘浩然,吴成新,刘英姿,姜素芳.HPLC法同时测定复方硝酸咪康唑乳凝胶中硝酸咪康唑及蛇床子素的含量[J].湖南师范大学学报(医学版),2011,8(4):84-86. |
| 作者姓名： | 陈艳玲 刘浩然 吴成新 刘英姿 姜素芳 |
| 作者单位： | 1. 长沙市中医院药剂科,湖南长沙,410002 2. 湖南大学化学化工学院,湖南长沙,410082 3. 湖南师范大学医学院,湖南长沙,410006 |
| 基金项目： | 湖南省教育厅资助科研项目 |
| 摘 要： | 目的:建立同时测定复方乳凝胶中硝酸味康唑及蛇床子素含量的方法.方法:采用超声波破乳、冰浴等方法对乳凝胶进行前处理去除杂质,提取药物.色谱条件为:Kromasil C18色谱柱(150mm×4.6mm,5?m)；流动相:有机相(乙腈:甲醇=65:35):0.2％醋酸-醋酸铵缓冲液]=86:14.流速为1mL/min；柱... |
| 关 键 词： | HPLC 硝酸咪康唑 蛇床子素 凝胶剂 |
Simultaneous assay of miconazole nitrate and osthole in compound cream by HPLC |
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| Chen Yanlin , Liu Haoran , Wu XinCheng , Liu Yingzi , Jiang Sufang.Simultaneous assay of miconazole nitrate and osthole in compound cream by HPLC[J].Journal of Hunan Normal University(Medical Science),2011,8(4):84-86. | |
| Authors: | Chen Yanlin Liu Haoran Wu XinCheng Liu Yingzi Jiang Sufang |
| Institution: | 1.Department of pharmaceutics,Traditional Chinese Medcine Hospital of Changsha,Hunan 410002,China; 2.College of chemical and chemisty engreening,Hunan University,Changsha 410082,China; 3.Medical College,Hunan Normal University,Changsha 410006,China) |
| Abstract: | Objective To establish a HPLC method for simultaneous assay 0f miconazole nitrate and osthole in compound cream.Methods Supersonic wave and ice bath was applied to destroyed the gel and extract the drug,with removing the impurities.The HPLC was carried out using a Kromasil C18(150×4.6 mm ID,5?m) analytical column at 30 ℃,with a mixture mobile phase Acetontrile:Methanol(65:35): 0.2%CH3COOH-CH3COONH4 buffer solution=86:14] at flow rate of 1 mL/min with the wavelength at 263 nm.Results The linear range of miconazole nitrate is 10.5~208.0 μg/mL,r=0.9998;The linear range of osthole is 7.0~112.0 μg/mL,r=0.9999.The mean recovery of them is 100.13±1.28% and 98.72±1.12%,respectively.Conclusion This method is suitable for the simultaneous assay of miconazole nitrate and osthole in cream for its high recovery and reproducibility. |
| Keywords: | HPLC Miconaxole nitrate Osthole Gels |
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