重组质粒hTERT-siRNA对胰腺癌细胞裸鼠皮下移植瘤生长抑制作用的研究

目的探讨重组质粒pSilencer4.1CMV neo-hTERT-siRNA对胰腺癌细胞BxPC-3裸鼠皮下移植瘤生长抑制作用.方法建立人原发性胰腺癌裸鼠皮下移植瘤模型,将荷瘤裸鼠随机分为4组：（1）肿瘤对照组,未作任何处理;（2）空载组,即pSilencer4.1-CMV neo空质粒组;（3）T1组,即重组质粒pSilencer4.1-CMV neo-hTERT1-siRNA组;（4）T2组,即重组质粒pSilencer4.1-CMVneo-hTERT2-siRNA组）.荷瘤裸鼠分组处理30d后,检测移植瘤体积及瘤重,HE染色光镜观察各组裸鼠皮下移植瘤及各脏器组织形态学,电镜观测各组移植瘤组织超微结构变化.结果 T1组及T2组肿瘤体积及瘤重均明显小于肿瘤对照组和空载组（P〈0.01）.T1组、T2组移植瘤组织癌细胞密度减少,部分癌巢发生程度不等片状坏死.T1组和T2组BxPC-3细胞超微结构发生了明显变化,细胞表面微绒毛减少或消失,部分线粒体肿胀,同时见胞质密度增加,可见凋亡小体.结论重组质粒T1和T2均能在裸鼠体内有效抑制BxPC-3胰腺癌细胞皮下移植瘤的生长,促进胰腺癌细胞凋亡,具有体内抗胰腺癌的作用。

Inhibiting Effects of Recombinant Plasmid hTERT-siRNA on Transplanted Tmour from Pancreatic Cancer Cells in Nude Mice

Objective To explore the inhibiting effects of recombinant plasmid pSilencer4.1CMV neo-hTERT-siRNA on transplanted tmour from BxPC-3 cells in nude mice.Methods The nude mice model of subcutaneous transplanted tumour from human primary pancreatic cancer was established.The nude mice with transplanted tumour were randomly divided into 4 groups：（1） tumour control group,nude mice received no treatment;（2） empty carrier group,nude mice received pSilencer4.1-CMVneo empty plasmid;（3） T1 group,nude mice received pSilencer4.1-CMVneo-hTERT1-siRNA;（4） T2 group,nude mice received pSilencer4.1-CMVneo-hTERT2-siRNA.Thirty days after treatment,the transplanted tumour volume and weight of nude mice was measured,the histopathological changes of transplanted tumour and organs were observed with light microscope after HE staining,the ultra structure of transplanted tumour was observed with electron microscope.Results The tumour volume and weight of nude mice in T1 group and T2 group were lower than those in tumour control group and empty carrier group.The density of cancer cells in transplanted tumour of nude mice in T1 group and T2 group was decreased,and necrosis of partial cancer nests was found.The ultrastructure of BxPC-3 cells in transplanted tumour of nude mice in T1 group and T2 group changed significantly,the microvillus on the surface of cells reduced or dispeared,some mitochondria swelled,the cytoplasm density increased,and apoptotic bodies were found.Conclusion Recombinant plasmids T1and T2 can inhibit the growth of subcutaneous transplanted tmour from BxPC-3 cells in nude mice,promote the apoptosis of BxPC-3 cells,and have anti-pancreatic cancer effects in vivo.