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| BCOP法评价滴眼液的角膜渗透性 | |
| 引用本文： | 谢珍,侯梦妮,孙叶丹,朱社敏,匡荣,李杨. BCOP法评价滴眼液的角膜渗透性[J]. 中国现代应用药学, 2017, 34(3): 390-392 |
| 作者姓名： | 谢珍 侯梦妮 孙叶丹 朱社敏 匡荣 李杨 |
| 作者单位： | 浙江省食品药品检验研究院, 杭州 310052,浙江工业大学, 杭州 310014,浙江省食品药品检验研究院, 杭州 310052,浙江省食品药品检验研究院, 杭州 310052,浙江省食品药品检验研究院, 杭州 310052,浙江省食品药品检验研究院, 杭州 310052 |
| 摘 要： | 目的 采用牛角膜混浊和渗透性试验（bovine corneal opacity and permeability，BCOP）方法对滴眼液的角膜渗透性进行评价。方法 利用BCOP法，以无酚红MEM培养液为介质，前室加滴眼液样品，于不同时间点后室取样，用紫外-可见分光光度法测定其苄达赖氨酸的含量，比较不同滴眼液样品的角膜渗透性。结果 不同厂家样品表现出不同的角膜渗透性，包括角膜累积渗透量、透过速率、表观渗透系数和稳态渗透速率均不同。结论 可用BCOP法进行眼用制剂的角膜渗透性研究。 |
| 关 键 词： | 牛角膜混浊和渗透性试验 苄达赖氨酸滴眼液 角膜渗透性 |
| 收稿时间： | 2016-09-11 |
| 修稿时间： | 2016-12-07 |
Study on the Application of BCOP in Corneal Permeability of Eye Drops |
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| XIE Zhen,HOU Mengni,SUN Yedan,ZHU Shemin,KUANG Rong and Li Yang. Study on the Application of BCOP in Corneal Permeability of Eye Drops[J]. The Chinese Journal of Modern Applied Pharmacy, 2017, 34(3): 390-392 | |
| Authors: | XIE Zhen HOU Mengni SUN Yedan ZHU Shemin KUANG Rong Li Yang |
| Affiliation: | Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China,Zhejiang University of Technology, Hangzhou 310014, China,Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China,Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China,Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China and Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China |
| Abstract: | OBJECTIVE To evaluate corneal permeability of eye drops by bovine corneal opacity and permeability (BCOP) assay. METHODS Used BCOP method, phenol red-free MEM medium was used as release medium, loaded eye drops into the anterior chamber, collected the samples form posterior chamber in different time point and detected the content of bendazac lysine by UV spectrophotography to compare corneal permeability of different eye drops. RESULTS Eye drops from different manufacturers showed different corneal permeability, including the outcomes of permeation amount, permeation rate, apparent permeability and steady-state rate of permeation had been verified. CONCLUSION The BCOP assay can be used in the study of corneal permeability of ophthalmic preparation. |
| Keywords: | bovine corneal opacity and permeability bendazac lysine eye drops corneal permeability |
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