Nutritional requirements of Streptococcus sanguis

The nutritional requirements of Streptococcus sanguis ATCC strains 10556, 10557 and 10558, Strep. mitis ATCC 9811, Strep. bovis ATCC 9809 and 37 strains isolated from the oral cavity in man and considered to be Strep. sanguis were studied under anaerobic conditions. Streptococcus sanguis ATCC 10558 and 23 of the oral strains required cysteine, glucose, pyridoxine, nicotinic acid, biotin, thiamin, riboflavin, pantothenic acid, ammonia and salts. One oral strain required in addition glutamic acid and Strep. sanguis ATCC 10556 isoleucine and valine. Strep. sanguis ATCC 10557 and 14 of the oral strains required arginine, cysteine, glutamic acid, histidine, glucose, nicotinic acid, biotin, thiamin, riboflavin, pantothenic acid, ammonia and salts. Strep. mitis ATCC 9811 had the same nutritional requirements. One oral strain required adenine in addition. Strep. bovis ATCC 9809 required cysteine, glucose, biotin, thiamin, ammonia and salts.