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| MCT1和LDHB在荷乳腺癌小鼠髓系抑制性细胞中的表达及意义 | |
| 引用本文： | 蓝瑞隆,张 娜,陈瑞庆. MCT1和LDHB在荷乳腺癌小鼠髓系抑制性细胞中的表达及意义[J]. 大连医科大学学报, 2017, 39(6): 532-535. DOI: 10.11724/jdmu.2017.06.03 |
| 作者姓名： | 蓝瑞隆 张 娜 陈瑞庆 |
| 作者单位： | 1. 福建医科大学附属第一医院中心实验室,福建福州350005;福建省个体化主动免疫治疗重点实验室,福建福州350005;2. 福建省血液病研究所福建省血液病学重点实验室福建医科大学附属协和医院,福建福州,350001 |
| 基金项目： | 基金项目：福建省卫计委青年课题（2016-1-60）；2016年福建医科大学启航基金（2016QH042） |
| 摘 要： | 目的 比较正常小鼠和荷乳腺癌小鼠骨髓中髓系抑制性细胞(MDSCs)的含量,探讨其产生免疫抑制功能的可能机制.方法 建立荷乳腺癌小鼠模型,利用BD FACSAria Ⅲ流式分选仪分选正常小鼠组和荷乳腺癌小鼠组骨髓中的MDSCs(CD11b+ Gr-1+),比较其含量;q-PCR法检测两组MDSCs中MCT1及LDHB的mRNA水平.结果 荷乳腺癌小鼠骨髓中MDSCs的比例为(48.92±3.13)％高于正常小鼠(25.13±1.95)％,差异有统计学意义(P ＜0.001);荷乳腺癌小鼠MDSCs中MCT1的mRNA相对表达量(0.010±0.005)为正常小鼠(0.003±0.001)的3.33倍,差异有统计学意义(P＜0.05);荷乳腺癌小鼠MDSCs中LDHB的mRNA相对表达量(0.008±0.002)为正常小鼠(0.004±0.001)的2.00倍,差异极显著(P＜0.01).结论 MDSCs在荷乳腺癌小鼠骨髓中的比例显著高于正常小鼠,其能量代谢关键分子MCTI、LDHB的表达也显著高于正常小鼠.MDSCs的能量代谢特点可能正是其发挥免疫抑制功能的重要基础. |
| 关 键 词： | MDSC MCT1 LDHB 能量代谢 免疫抑制 |
Expression and significance of MCT1 and LDHB in MDSCs from bone marrow of breast cancer bearing mice |
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| Affiliation: | Central Laboratory of the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China; Fujian Key Laboratory of Individualized Active Immunotherapy,Fuzhou 350005,China;,Fujian Insitute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital,Fuzhou 350001,China and Central Laboratory of the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China; Fujian Key Laboratory of Individualized Active Immunotherapy,Fuzhou 350005,China; |
| Abstract: | Objective To test the MDSCs ratios in bone marrow from normal and breast cancer bearing mice,and compare the mRNA levels of the key energy metabolism molecules-MCT1/LDHB and explore the possible mechanism of immunosuppression in MDSCs.Methods MDSCs (CD1 1 b + Gr-1 +) in bone marrow was sorted by BD FACSAria Ⅲ.MCT1/LDHB mRNA levels were detected with q-PCR.Results The MDSCs ratio (19.77 ± 4.77) ％ in breast cancer bearing mice was much higher than that (0.84 ± 0.18) ％ in normal group (P ＜ 0.001).Both MCT1 and LDHB mRNA of MDSCs in tumor bearing group were significantly higher than that in normal group (P ＜0.05 and P ＜0.01,respectively).Conclusions The energy metabolism could play an role in the immunosuppression of MDSCs. |
| Keywords: | MDSC MCT1 LDHB energy metabolism immunosuppression |
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