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DNA repair gene expression in biological tissues exposed to low-intensity infrared laser |
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| Authors: | Adenilson de Souza da Fonseca Andre Luiz Mencalha Vera Maria Araújo de Campos Samara Cristina Ferreira Machado Antonio Augusto de Freitas Peregrino Mauro Geller Flavia de Paoli |
| Affiliation: | 1. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua S?o Francisco Xavier, 524, Maracan?, Rio de Janeiro, 20550900, Brazil 2. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro, 20211040, Brazil 3. Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense, Rua Outeiro de S?o Jo?o Batista, s/n, Niterói, 24020-140, Brazil 4. Setor de Facomatoses do Servi?o de Genética Clínica do IPPMG, Universidade Federal do Rio de Janeiro, Avenida Brigadeiro Trompowsky, Rio de Janeiro, 21949590, Brazil 5. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Louren?o Kelmer, s/n-Campus Universitário, S?o Pedro, Juiz de Fora, Minas Gerais, 36036900, Brazil |
| Abstract: | Special properties of laser light have led to its usefulness in many applications in therapy. Excitation of endogenous chromophores in biotissues and generation of free radicals could be involved in its biological effects. DNA lesions induced by free radicals are repaired by base excision repair pathway. In this work, we evaluated the expression of APE1 and OGG1 genes related to repair of DNA lesions induced by free radicals. Skin and muscle tissues of Wistar rats were exposed to low-intensity infrared laser at different fluences and frequencies. After laser exposition of 1 and 24 h, tissue samples were withdrawn for total RNA extraction, cDNA synthesis, and evaluation of APE1 and OGG1 gene expression by quantitative polymerase chain reaction. Data obtained show that laser radiation alters the expression of APE1 and OGG1 mRNA differently in skin and muscle tissues of Wistar rats depending of the fluence, frequency, and time after exposure. Our study suggests that low-intensity infrared laser affects expression of genes involved in repair of DNA lesions by base excision repair pathway. |
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