加味生脉饮含药血清对非小细胞肺癌细胞迁移及黏附的影响

目的：研究加味生脉饮含药血清对非小细胞肺癌细胞迁移、黏附的影响，并探讨其分子机制。方法以生药量18 g／kg灌胃家兔，制备加味生脉饮含药血清，同容积生理盐水灌胃制备空白血清。体外培养细胞，收集对数期生长的细胞，设10％空白血清组，2．5％、5％、10％含药血清组4个组。 Transwell和黏附实验分别检测加味生脉饮含药血清对非小细胞肺癌细胞迁移及黏附的影响；RT-PCR测定经含药血清处理后细胞中N钙连素（ N-cad）及波形蛋白（ vimentin）基因表达的变化。结果随着含药血清浓度的增高，迁移到膜下表面的细胞数减少，5％和10％含药血清组迁移率仅为25．64％和13．31％（P＜0．01）。随着含药血清浓度增高和作用时间延长，异质黏附率降低，且黏附率和血清浓度及作用时间呈负相关；同时N-cad、vimentin基因的表达量下降，呈浓度与时间相关性。结论加味生脉饮含药血清对非小细胞肺癌细胞有抑制迁移和降低异质黏附作用，其分子机制与影响上皮间质转化有关。

Effects of serum containing modified Shengmai liquid on migration and adhesion of non small cell lung cancer cells

Objective To study the effects and mechanism of serum containing modified Shengmai liquid ( MSL) on migration and adhesion of non small cell lung cancer ( NSCLC) cells.Methods Rabbit ser-um containing MSL was prepared by intragastric administration ( i.g.) with MSL at the dose of 18 g/kg and control serum was prepared by i.g.normal saline.NSCLC cells were cultured, and those in logarith-mic growth phase collected were divided into four groups:10%control serum(CS) group, 2.5%, 5%, 10%MSL serum group.Migration cells were detected by using Transwell invasion assay, and adhension rate was measured by using matrigel adhensive assay.The expressions of N-cad and vimentin were de-termined by RT-PCR.Results The number of migration cells decreased as the concentration of serum containg MSL increased:migration rate of 5%MSL group was 25.64%and that of 10%MSL group was 13.31%(P<0.01).As the concentration of serum containing MSL increased and the duration of treat-ment prolonged, heterogeneous adhesion rate decreased and was negatively correlated with serum concen-tration and duration;while the gene expressions of N-cad and vimentin decreased in dose-and time-de-pendent manner.Conclusion The mechanism of inhabitation migration and reduction heterogeneity ad-hension of MSL on NSCLC cells would be related to the epithelial-mesenchymal transition.