急性肝功能衰竭大鼠肝组织iNOS的表达及其意义

目的检测急性肝功能衰竭（ALF）大鼠肝组织诱导型一氧化氮合酶（iNOS）的表达,探讨其在ALF中的意义。方法 SD大鼠随机分为3组：对照组给予生理盐水（NS）1ml腹腔注射;实验组予D-氨基半乳糖联合内毒素腹腔注射,复制ALF大鼠模型,分别在6、12h处死大鼠即为ALF6h组（D-6组）和ALF12h组（D-12组）,门静脉采血检测肝功能;留取肝组织标本,免疫组织化学方法检测iNOS,阳性产物灰度值应用图像分析软件半定量分析。结果对照组肝组织iNOS无表达;实验组6、12h肝脏病理呈进展性,肝组织iNOS沿中央静脉及残存小血管高表达,其平均灰度值在D-6组为81.02±5.86,D-12组为130.71±7.16,两组差异有统计学意义（P〈0.05）。结论 ALF大鼠肝组织iNOS高表达;缺氧、微循环障碍可能是诱导iNOS表达的重要因素。

The Expression and Significance of INOS in Liver Tissue of Rats with Acute Liver Failure

Objective To study the significance of inducible nitric oxide synthase（iNOS） in acute liver failure（ALF）,by detecting the expression of it in liver of rats with ALF.Methods Thirty rats were randomly divided into three groups,in the control group were injected intraperitoneally with 1 ml normal saline.To induce ALF,rats were injected intraperitoneally with D-galactosamine（D-GalN） and lipopolysaccharide（LPS）.The rats were executed atfter the 6th and 12th hour,respectively,named the D-6 group and the D-12 group.Blood was taken from portal vein to detect liver function.The liver tissue specimens were taken to detect the expression of iNOS by immunohisto-chemistry assay.Image analysis system was used to analyze the average gray value.Results There were no stained iNOS immunoreactive cells in the control group.The pathological change is progressive at the 6th and 12th hour in the experimental groups,in which high expression of iNOS alongside the central veins and residual venules was observed.The mean gray value of iNOS was 81.02 ± 5.86 in the D-6 group and 130.71 ± 7.16 in the D-12 group,respectively（P 0.05）.Conclusion High expression of iNOS is observed in the liver tissue of rats with ALF.Hypoxia and microcirculation disorder may be the important reason that leads to its expression.