脑温对局灶脑缺血再灌注损伤氨基酸含量的影响

目的研究轻度高温、亚低温对局灶脑缺血EAA和IAA的影响。方法建立大鼠可复流MCA闭塞模型，诱导目标脑温，用HPLC荧光法检测脑组织Glu、Asp、Gly、GABA含量。结果轻度高温组Glu、Asp、Gly明显增高，GABA短暂增高后下降；亚低温组Glu、Asp、Gly降低,GABA持续增高。结论轻度高温促进［Glu］×［Gly］/［GABA］比值增加，在持续增加Glu“兴奋毒性”方面起重要作用；亚低温使［Glu］×［Gly］/［GABA］比值下降，在降低Glu“兴奋毒性”方面起重要作用。轻度高温不利于；亚低温有利于“抑制性保护”机制的建立。

The effects of different brain temperature on amino acid concentration during reperfusion injury following focal cerebral ischemia

Objective　To research the effects of mild hyperthermia and mild hypothermia on excitatory amino acid(EAA) and inhibitory amino acid(IAA) respectively.Methods　Test the concentration of glutamate(Glu)，aspartate(Asp)，glycine(Gly) and GABA respectively by using HPLC after establishing of model of reflowable cerebral middle artery occlusion of rats and inducing target brain temperature.Results　The concentration of Glu,Asp,Gly increased markedly, and that of GABA increased significantly and temporarily, Then decreased rapidly in mild hyperthermia group, The concentration of Glu,Asp,Gly decreases significantly and that of GABA increased markedly and continuously in mild hypothermia group.Conclusions　 Mild hyperthermia increases the value of ratio of ［Glu］×［Gly］/［GABA］ and plays an important role in continuously increasing excitatory toxicity of Glu, mild hypothermia decreases the value of ratio of ［Glu］×［Gly］/［GABA］ and serves as a significant part in reducing excitatory toxicity of Glu.