大肠癌患者血浆miR-106a的表达及意义

目的研究血浆miR-106a在大肠癌患者中的表达及其与临床病理特征的关系，分析其诊断价值。方法选取3例大肠癌组
织及配对正常粘膜组织进行miRNA芯片检测，筛选出大肠癌组织中异常表达的miRNA；收集50例首治的大肠癌患者的血浆标
本和47例健康志愿者的血浆作为对照，提取血浆中总RNA，运用荧光定量PCR方法检测miR-106a在大肠癌和正常人血浆中的
表达情况。同时收集患者术后7 d血浆标本40例，分析术前、术后miR-106a的表达变化。分析术前血浆miR-106a表达与临床
病理特征间的关系，并对受试者行ROC分析。结果miRNA芯片检测大肠癌组织中miR-106a的表达较正常粘膜明显增加；与
健康对照组相比，miR-106a在大肠癌患者血浆中表达量显著上调（P=0.012），术后患者血浆miR-106a的表达较术前明显降低
（P<0.01）。术前血浆miR-106a的表达与大肠癌患者淋巴结转移、TNM分期及分化程度均无关（P>0.05)。miR-106a 所得ROC
曲线下面积为0.661(95% CI∶0.55~0.772)，区别大肠癌和正常人的敏感性为62.3%，特异性为68.2%。结论血浆miR-106a在大
肠癌患者中表达上调，可能对大肠癌的诊断具有一定临床价值。

Expression of plasma miR-106a in colorectal cancer and its clinical significance

Objective To detect plasma miR-106a level in patients with colorectal cancer (CRC) and analyze its correlation to the
clinicopathological features and disease diagnosis. Methods miRNA expression profiling was performed using miRNA
microarray chip for 3 colorectal adenocarcinoma samples and matched normal tissues. Plasma samples was collected from 50
colorectal cancer patients for quantitative analysis of miR-106a using real-time RT-PCR using 47 plasma samples from healthy
volunteer as the control. Forty plasma samples were collected from these patients 7 days after operation to examine the
changes in miR-106a expression. Results miR-106a was differentially expressed in colorectal adenocarcinoma compared to
normal tissues. The plasma levels of miR-106a expression were significantly higher in the cancer patients than in the healthy
control group (P=0.012). miR-106a expression significantly decreased after the operation compared with its preoperative level
(P<0.01), and no correlation was found between preoperative plasma miR-106a and the clinicopathological features including
lymph node metastasis and TNM stage (P>0.05). miR-106a showed a receiver operating characteristic (ROC) curve area of
66.1%, a sensitivity of 62.3%, and a specificity of 68.2% in discriminating colorectal cancer patients from the control subjects.
Conclusion plasma miR-106a is up-regulated in CRC patients, suggesting its potential value for the diagnosis of CRC.