射频消融诱导兔VX2肝脏移植瘤细胞凋亡

目的　探讨射频消融 (RFA)对兔VX2 肝脏移植瘤的治疗机制。方法　通过VX2 肿瘤组织混悬液肝内注入法建立兔肝脏肿瘤模型 ,随机分为实验组及对照组 ,分别予以RFA治疗及假性处理 ,于治疗结束后 0、12h、1、2、4、8、16d共 7个时间点分别切取肿瘤中心组织及边缘组织为检测标本 ,进行HE染色、透射电镜检查并运用TUNEL法检测肿瘤细胞凋亡情况。结果　治疗组毁损区边缘部组织中有大量阳性细胞 ,中心部分亦有较多的阳性细胞 ,两者凋亡细胞指数差异非常显著 (P <0 .0 1)。对照组标本中仅发现极少数阳性细胞 ,且其中心区与边缘区无统计学差异 ,其凋亡细胞指数与治疗组标本相比 ,差异均非常显著 (P <0 .0 1)。于不同时间点观察 ,治疗组细胞凋亡高峰在 2 4h左右 ,而对照组细胞凋亡则无明显时间分布特征。结论　诱导肿瘤细胞凋亡是射频消融治疗肝脏恶性肿瘤的一项重要机制。其发生的部位主要是肿瘤周边区 ,时间高峰在RFA作用后 2 4h左右。

Apoptosis induced by radiofrequency ablation in the rabbit liver tumor

Objective To explore the therapeutic mechanism of RFA on the rabbit VX 2 hepato-carcinoma. Methods The rabbit hepatic neoplasm model was established by injecting the VX 2 tumor constitution mass into the liver of the rabbits. The animals were randomly divided into two groups: the experimental group and the control group. The former was treated by RFA, and the later was treated with the same operation but RFA. The central tissue and the marginal tissue of the ablation region was cut at 0 h, 12 h, 1 d, 2 d, 4 d, 8 d and 16 d after RFA, respectively, and the HE dyeing, the transmission electron microscope examination and the TUNEL analysis were used to detect their apoptotic cells. Results A great deal positive cells were found in the central tissue of the experimental group and more positive cells were also found in the ringle tissue, and there was significant difference (P<0.01). Few positive cells were found in the control group, and there was no difference between their ringle and central tissues. The difference between the apoptotic index of the experimental group and the control group was very significant (P<0.01). The apoptotic peak of the experimental group appeared at 24 h after RFA. The apoptotic cell number of the control group did not vary with time. Conclusions To induce malignant tumor cellular apoptosis it is an important mechanism of treating hepatic neoplasm by RFA, the main apoptotic lies in the ringle region of the tumor and the apoptotic peak appears at 24 h after RFA.