白介素17在慢性阻塞性肺疾病大鼠肺组织中的表达及与气道炎症及重塑的关系

目的 探讨白介素17(IL-17)在慢性阻塞性肺疾病(COPD)大鼠气道炎症及重塑中的作用.方法 采用被动吸烟80 d来制备大鼠COPD模型.支气管肺泡灌洗计数支气管肺泡灌洗液(BALF)中细胞计数和百分比;采用半定量图像分析法测量小气道管壁和平滑肌层厚度;免疫组织化学法检测肺组织IL-17的表达;酶联免疫吸附试验法检...

Expression of interleukin-17 in lung tissue of rats with chronic obstructive pulmonary disease and its correlation with airway inflammation and remodeling

Objective To study the role of interleukin-17 (IL-17) in the airway inflammation and remodeling of rats with chronic obstructive pulmonary disease (COPD). Methods Rat models of COPD were replicated by passive smoking for 80 days. Total cell counts and neutrophil counts in bronchoalveolar lavage fluid (BALF) were examined. The thickness of the airway wall and the smooth muscle layer of the small airway were measured by means of image analyzer. The expression of IL-17 in rat lung tissue was detected by immunohistochemistry. The concentrations of IL-17 in lung tissues and tumor necrosis factorα(TNF-α) in BALF and serum were measured with enzyme-linked immunosorbent assay. Results Rat models of COPD were replicated successfully by passive smoking. IL-17 was mainly expressed in airway epithelial cells of COPD rats. The concentrations of IL-17 in lung tissue were significantly higher in the COPD group than in the normal group( P <0. 01). In the COPD group,IL-17 concentration of lung tissue was positively correlated with the neutrophil counts and TNF-α level in BALF( r =0. 65, P <0. 05. r =0. 76, P <0. 01), it also tended to be positively correlated with the thickness of the airway wall and the smooth muscle layer of the small airwayC r =0. 89, P <0. 01. r =0. 80, P <0. 01 ). Conclusions IL-17 probably recruits neutrophils into airways by enhancing the expression of TNF-α,and it may play a role in the airway inflammation and remodeling of COPD.